化学
丁酰胆碱酯酶
荧光
杀虫剂
环境化学
纳米技术
乙酰胆碱酯酶
有机化学
酶
生态学
阿切
物理
材料科学
量子力学
生物
作者
Zhimin Zhang,Jingkang Li,Bin Yang,Minglong Ma,Xiangdong Ding,Hui Su,Pinyi Ma,Daqian Song,Ziwei Zhang
出处
期刊:Talanta
[Elsevier]
日期:2024-07-18
卷期号:279: 126587-126587
被引量:1
标识
DOI:10.1016/j.talanta.2024.126587
摘要
The toxicity of organophosphorus pesticides (OPs) can catastrophically cause liver cell damage and inhibit the catalytic activity of cholinesterase. We designed and synthesized a near-infrared fluorescent probe HP-LZB with large Stokes shift which can specifically identify and detect butyrylcholinesterase (BChE) and visually explore the interaction between OPs and endogenous BChE in living cells. Fluorescence was turned on when HP-LZB was hydrolyzed into HP-LZ in the presence of BChE, and OPs could inhibit BChE's activity resulting in a decrease of fluorescence. Six OPs including three oxon pesticides (paraoxon, chlorpyrifos oxon and diazoxon) and their corresponding thion pesticides (parathion, chlorpyrifos and diazinon) were investigated. Both in vitro and cell experiments indicated that only oxon pesticides could inhibit BChE's activity. The limits of detection (LODs) of paraoxon, chlorpyrifos oxon and diazoxon were as low as 0.295, 0.007 and 0.011 ng mL
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