Biocompatibility of Novel Marine Collagen on Periodontal Ligament Fibroblasts: A Pathway to Enhanced Regenerative Therapies

ABSTRACT Body wall of sea cucumber Bohadschia bivittate contains a protein consisting of highly insoluble collagen fibers. We aimed to evaluate the biocompatibility and cytotoxicity of nonirradiated or γ ‐irradiated pepsin soluble collagen (PSC) extracted from Bohadschia bivittata on human periodontal ligament fibroblasts cells. The MTT assay showed significant increase in the cell viability values indicating that PSC is noncytotoxic. Further, nonirradiated PSC showed higher cell viabilities values than γ ‐irradiated PSC at all concentration, especially at an optimal dilution of 25% ( p < 0.05). This exploratory study suggests that PSC from Bohadschia bivittata could be utilized as a novel biomaterial in periodontal regenerative therapies.