In Vivo Silencing of Regulatory Elements Using a Single AAV-CRISPRi Vector

HomeCirculation ResearchAhead of PrintIn Vivo Silencing of Regulatory Elements Using a Single AAV-CRISPRi Vector No AccessResearch ArticleRequest AccessAboutView PDFSections ToolsAdd to favoritesDownload citationsTrack citationsPermissions ShareShare onFacebookTwitterLinked InMendeleyReddit Jump toNo AccessResearch ArticleRequest AccessIn Vivo Silencing of Regulatory Elements Using a Single AAV-CRISPRi Vector P. Laurette, C. Cao, D. Ramanujam, M. Schwaderer, T. Lueneburg, S. Kuss, L. Weiss, R. Dilshat, E.E.M. Furlong, F. Rezende, S. Engelhardt and R. Gilsbach P. LauretteP. Laurette https://orcid.org/0000-0002-8941-612X Institute of Experimental Cardiology, Heidelberg University Hospital, Germany (P.L., C.C., T.L., S.K., R.G.). DZHK (German Center of Cardiovascular Research) Partner Site Rhein/Main, Mannheim, Germany (P.L., C.C., D.R., T.L., S.K., L.W., F.R., S.E., R.G.). Institute of Cardiovascular Physiology, Frankfurt University, Germany (P.L., C.C., L.W., F.R., R.G.). , C. CaoC. Cao https://orcid.org/0000-0001-8559-3535 Institute of Experimental Cardiology, Heidelberg University Hospital, Germany (P.L., C.C., T.L., S.K., R.G.). DZHK (German Center of Cardiovascular Research) Partner Site Rhein/Main, Mannheim, Germany (P.L., C.C., D.R., T.L., S.K., L.W., F.R., S.E., R.G.). Institute of Cardiovascular Physiology, Frankfurt University, Germany (P.L., C.C., L.W., F.R., R.G.). , D. RamanujamD. Ramanujam https://orcid.org/0000-0003-4854-6355 DZHK (German Center of Cardiovascular Research) Partner Site Rhein/Main, Mannheim, Germany (P.L., C.C., D.R., T.L., S.K., L.W., F.R., S.E., R.G.). , M. SchwadererM. Schwaderer Institute of Clinical and Experimental Pharmacology and Toxicology, University of Freiburg, Germany (M.S.). , T. LueneburgT. Lueneburg Institute of Experimental Cardiology, Heidelberg University Hospital, Germany (P.L., C.C., T.L., S.K., R.G.). DZHK (German Center of Cardiovascular Research) Partner Site Rhein/Main, Mannheim, Germany (P.L., C.C., D.R., T.L., S.K., L.W., F.R., S.E., R.G.). , S. KussS. Kuss Institute of Experimental Cardiology, Heidelberg University Hospital, Germany (P.L., C.C., T.L., S.K., R.G.). DZHK (German Center of Cardiovascular Research) Partner Site Rhein/Main, Mannheim, Germany (P.L., C.C., D.R., T.L., S.K., L.W., F.R., S.E., R.G.). , L. WeissL. Weiss https://orcid.org/0009-0005-4574-8455 DZHK (German Center of Cardiovascular Research) Partner Site Rhein/Main, Mannheim, Germany (P.L., C.C., D.R., T.L., S.K., L.W., F.R., S.E., R.G.). Institute of Cardiovascular Physiology, Frankfurt University, Germany (P.L., C.C., L.W., F.R., R.G.). , R. DilshatR. Dilshat https://orcid.org/0000-0002-2126-2902 European Molecular Biology Laboratory, Genome Biology Unit, Heidelberg, Germany (R.D., E.E.M.F.). , E.E.M. FurlongE.E.M. Furlong https://orcid.org/0000-0002-9544-8339 European Molecular Biology Laboratory, Genome Biology Unit, Heidelberg, Germany (R.D., E.E.M.F.). , F. RezendeF. Rezende DZHK (German Center of Cardiovascular Research) Partner Site Rhein/Main, Mannheim, Germany (P.L., C.C., D.R., T.L., S.K., L.W., F.R., S.E., R.G.). Institute of Cardiovascular Physiology, Frankfurt University, Germany (P.L., C.C., L.W., F.R., R.G.). , S. EngelhardtS. Engelhardt https://orcid.org/0000-0001-5378-8661 DZHK (German Center of Cardiovascular Research) Partner Site Rhein/Main, Mannheim, Germany (P.L., C.C., D.R., T.L., S.K., L.W., F.R., S.E., R.G.). Institute of Pharmacology and Toxicology, Technical University of Munich, Germany (D.R., S.E.). and R. GilsbachR. Gilsbach Correspondence to: R. Gilsbach, Institute of Experimental Cardiology, Heidelberg University Hospital, Heidelberg, Germany https://orcid.org/0000-0002-0895-1535 Institute of Experimental Cardiology, Heidelberg University Hospital, Germany (P.L., C.C., T.L., S.K., R.G.). DZHK (German Center of Cardiovascular Research) Partner Site Rhein/Main, Mannheim, Germany (P.L., C.C., D.R., T.L., S.K., L.W., F.R., S.E., R.G.). Institute of Cardiovascular Physiology, Frankfurt University, Germany (P.L., C.C., L.W., F.R., R.G.). Institute of Pharmacology and Toxicology, Technical University of Munich, Germany (D.R., S.E.). Originally published22 Dec 2023https://doi.org/10.1161/CIRCRESAHA.123.323854Circulation Research. 2023;0FootnotesFor Sources of Funding and Disclosures, see page xxx.Correspondence to: R. Gilsbach, Institute of Experimental Cardiology, Heidelberg University Hospital, Heidelberg, Germany eLetters(0) eLetters should relate to an article recently published in the journal and are not a forum for providing unpublished data. Comments are reviewed for appropriate use of tone and language. Comments are not peer-reviewed. Acceptable comments are posted to the journal website only. Comments are not published in an issue and are not indexed in PubMed. Comments should be no longer than 500 words and will only be posted online. References are limited to 10. Authors of the article cited in the comment will be invited to reply, as appropriate. Comments and feedback on AHA/ASA Scientific Statements and Guidelines should be directed to the AHA/ASA Manuscript Oversight Committee via its Correspondence page. Sign In to Submit a Response to This Article Previous Back to top Next FiguresReferencesRelatedDetails Advertisement Article Information Metrics © 2023 American Heart Association, Inc.https://doi.org/10.1161/CIRCRESAHA.123.323854PMID: 38131200 Originally publishedDecember 22, 2023 Keywordsepigenesis, geneticgene editinggene silencinggenetic therapygenomicsPDF download Advertisement Subjects Basic Science Research Epigenetics