已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Investigating the molecular mechanism of Qizhu anticancer prescription in inhibiting hepatocellular carcinoma based on high-resolution mass spectrometry and network pharmacology

肝细胞癌 机制(生物学) 质谱法 药理学 药方 医学 化学 传统医学 癌症研究 色谱法 哲学 认识论
作者
Jialing Sun,Mengqing Ma,Xin Zhong,Jing Li,Jinyu Yi,Renjie Zhang,Xingning Liu,Lanfen Peng,Xinfeng Sun,Wenxing Feng,Rui Hu,Qi Huang,Minling Lv,Kongli Fan,Xiaozhou Zhou
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:328: 117985-117985 被引量:3
标识
DOI:10.1016/j.jep.2024.117985
摘要

Of all primary liver cancer cases, hepatocellular carcinoma (HCC) accounts for about 85%. Most patients with HCC receive a diagnosis in the medium-to-late stages or with chronic liver disease, have lost the opportunity for radical treatment, such as surgical resection, and their 5-year survival rate is low. Qizhu Anticancer Prescription (QZACP) is an empirical formula composed of traditional Chinese herbs that can clinically relieve HCC symptoms, inhibit the progression of HCC, reduce recurrence rate, and prolong survival; however, its exact mode of action remains unknown. This study's purpose was to investigate the mode of action of QZACP in the prevention and treatment of HCC. Initially, drug components in the QZACP decoction were analyzed using high-resolution mass spectrometry. A subcutaneous tumor xenograft model in nude mice was constructed to further analyze the active components of QZACP that had entered tumor tissues through oral administration. Potential targets of QZACP in the prevention and treatment of HCC were identified and then confirmed in vivo via network pharmacology and molecular docking. In addition, regulatory effects of QZACP on HCC cell proliferation and the cell cycle were detected using a CCK-8 assay and flow cytometry. High-resolution mass spectrometry revealed that the QZACP decoction contained deacetyl asperulosidic acid methyl ester (DAAME), paeoniflorin, calycosin-7-glucoside, liquiritin, glycyrrhizic acid, astragaloside IV, saikosaponin A, curdione, and atractylenolide II. In nude mice, QZACP could effectively inhibit the growth of subcutaneous tumors, where DAAME, paeoniflorin, liquiritin, and glycyrrhizic acid could enter liver cancer tissues after oral administration. Among these, DAAME was the most highly expressed in HCC tissues and may be an important active component of QZACP for inhibiting HCC. Utilizing network pharmacology, the targets of action of these four drug components were identified. After verification using western blotting, STAT3, VEGFA, JUN, FGF2, BCL2L1, AR, TERT, MMP7, MMP1, ABCB1, CA9, and ESR2 were identified as targets of QZACP inhibition in HCC. In vitro experiments revealed that QZACP inhibited the proliferation of HCC cells while inducing G0/G1 phase cell cycle arrest. In vivo experiments demonstrated that DAAME significantly inhibited HCC growth. After intersection of the 24 DAAME targets predicted using network pharmacology with the 435 HCC disease targets, only CA9 was identified as a DAAME-HCC crossover target. Molecular docking results revealed that the binding site of DAAME and CA9 had good stereo-complementarity with a docking score of −8.1 kcal/mol. Western blotting and immunohistochemical results also confirmed that DAAME significantly decreased CA9 protein expression in HCC. QZACP inhibits HCC by reducing the expression of STAT3, VEGFA, JUN, FGF2, BCL2L1, AR, TERT, MMP7, MMP1, ABCB1, CA9, and ESR2. DAAME may be an important active component of QZACP for the prevention and treatment of HCC, inhibiting it by targeting the expression of CA9.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
零知识完成签到 ,获得积分10
2秒前
伽拉发布了新的文献求助10
8秒前
洸彦完成签到 ,获得积分10
10秒前
CipherSage应助G1997采纳,获得10
10秒前
Ethan完成签到 ,获得积分0
11秒前
11秒前
Rondab应助科研通管家采纳,获得10
12秒前
科研通AI2S应助科研通管家采纳,获得10
13秒前
13秒前
CipherSage应助科研通管家采纳,获得10
13秒前
Rondab应助科研通管家采纳,获得10
13秒前
Rondab应助科研通管家采纳,获得10
13秒前
SciGPT应助科研通管家采纳,获得10
13秒前
SciGPT应助科研通管家采纳,获得10
13秒前
13秒前
明钟达完成签到 ,获得积分10
16秒前
domingo发布了新的文献求助20
16秒前
HughWang完成签到,获得积分10
19秒前
嘟嘟豆806完成签到 ,获得积分10
21秒前
24秒前
火星完成签到 ,获得积分10
24秒前
26秒前
研友_Zlx3aZ发布了新的文献求助10
28秒前
认真的三问完成签到,获得积分20
30秒前
kyfbrahha完成签到 ,获得积分10
31秒前
31秒前
maleo发布了新的文献求助30
34秒前
domingo完成签到,获得积分10
35秒前
一直向前发布了新的文献求助10
35秒前
希望天下0贩的0应助Unicorn采纳,获得50
36秒前
toto完成签到 ,获得积分10
36秒前
宣灵薇完成签到,获得积分0
37秒前
37秒前
41秒前
大模型应助黎乐荷采纳,获得10
42秒前
福福发布了新的文献求助10
44秒前
cc完成签到 ,获得积分10
44秒前
大帅比完成签到 ,获得积分10
46秒前
46秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
Aktuelle Entwicklungen in der linguistischen Forschung 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989957
求助须知:如何正确求助?哪些是违规求助? 3532034
关于积分的说明 11256000
捐赠科研通 3270880
什么是DOI,文献DOI怎么找? 1805070
邀请新用户注册赠送积分活动 882252
科研通“疑难数据库(出版商)”最低求助积分说明 809216