烟曲霉
氧化苦参碱
真菌性角膜炎
上睑下垂
炎症
角膜炎
微生物学
泊沙康唑
曲菌病
曲霉
化学
伏立康唑
抗真菌
医学
生物
免疫学
药理学
皮肤病科
炎症体
作者
Weiyi Liu,Xue Tian,Lingwen Gu,Bing Yu,Ziyi Wang,Menghui Chi,Jing Lin,Qian Wang,Guibo Liu,Bing Yu,Li Cui
标识
DOI:10.1016/j.exer.2024.109830
摘要
Fungal keratitis (FK) is a refractory keratitis caused by excessive inflammation and fungal damage. Excessive inflammation can lead to tissue damage and corneal opacity, resulting in a poor prognosis for FK. Oxymatrine (OMT) is a natural alkaloid, which has rich pharmacological effects, such as antioxidant and anti-inflammation. However, its antifungal activity and the mechanism of action in FK have not been elucidated. This study confirmed that OMT suppressed Aspergillus fumigatus growth, biofilm formation, the integrity of fungal cell and conidial adherence. OMT not only effectively reduced corneal fungal load but also inflammation responses. OMT lessened the recruitment of neutrophils and macrophages in FK. In addition, OMT up-regulated the expression of Nrf2 and down-regulated the expression of IL-18, IL-1β, caspase-1, NLRP3 and GSDMD. Pre-treatment with Nrf2 inhibitor up-regulated the expression of IL-1β, IL-18, caspase-1, NLRP3 and GSDMD supressed by OMT. In conclusion, OMT has efficient anti-inflammatory and antifungal effects by suppressing fungal activity and restricting pyroptosis via Nrf2 pathway. OMT is considered as a potential option for the treatment of FK.
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