Identification of distinct immune infiltration and potential biomarkers in patients with liver ischemia-reperfusion injury

免疫系统 基因 生物 炎症 渗透(HVAC) CD8型 基因表达 免疫学 癌症研究 遗传学 物理 热力学
作者
Zhangliu Jin,Meng Dou,Weihui Peng,Boen Xiao,Jinjin Liu,Wen Meng,Wei Liu
出处
期刊:Life Sciences [Elsevier]
卷期号:327: 121726-121726 被引量:3
标识
DOI:10.1016/j.lfs.2023.121726
摘要

To identify alterations of specific gene expression, immune infiltration components, and potential biomarkers in liver ischemia-reperfusion injury (IRI) following liver transplantation (LT).GSE23649 and GSE151648 datasets were obtained from the Gene Expression Omnibus (GEO) database. To determine the differentially expressed genes (DEGs), we utilized the R package "limma". We also identify the infiltration of different immune cells through single-sample gene-set enrichment analysis (ssGSEA). Furthermore, we utilized LASSO logistic regression to select feature genes and Spearman's rank correlation analysis to determine the correlation between these genes and infiltrating immune cells. Finally, the significance of these feature genes was confirmed using a mouse model of hepatic IRI.A total of 17 DEGs were acquired, most of which were associated with inflammation, apoptosis, cell proliferation, immune disorders, and cellular response. 28 immune cell types were determined using ssGSEA. 5 feature genes (ADM, KLF6, SERPINE1, SLC20A1, and HBB) were screened using LASSO analysis, but the HBB gene was ultimately excluded due to the lack of statistical significance in the GSE151648 dataset. These 4 feature genes were predominantly related to immune cells. Finally, 15 significantly distinctive types of immune cells between the control and IRI groups were verified.We unveiled that macrophages, dendritic cells (DCs), neutrophils, CD4 T cells, and other immune cells infiltrated the IRI that occurred after LT. Moreover, we identified ADM, KLF6, SERPINE1, and SLC20A1 as potential biological biomarkers underlying IRI post-transplant, which may improve the diagnosis and prognosis of this condition.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
科研通AI2S应助胡萝卜糊了采纳,获得10
刚刚
1秒前
1秒前
强健的雅绿完成签到,获得积分10
2秒前
2秒前
3秒前
焱冰完成签到,获得积分10
3秒前
传奇3应助不要引力采纳,获得10
3秒前
3秒前
chengyi发布了新的文献求助30
4秒前
LSY应助我是大皇帝采纳,获得10
4秒前
Shawn_54发布了新的文献求助10
5秒前
cctv18应助wkkk采纳,获得10
5秒前
有机Fans发布了新的文献求助10
5秒前
6秒前
自不惊扰完成签到,获得积分10
6秒前
Li完成签到,获得积分10
7秒前
FashionBoy应助山月采纳,获得10
7秒前
7秒前
体贴静竹完成签到 ,获得积分10
8秒前
8秒前
乐观的从梦完成签到,获得积分10
8秒前
神勇松完成签到,获得积分10
9秒前
10秒前
10秒前
10秒前
ld发布了新的文献求助10
11秒前
11秒前
可靠的南霜完成签到 ,获得积分10
13秒前
天宇发布了新的文献求助10
13秒前
13秒前
淡然天真发布了新的文献求助10
13秒前
14秒前
14秒前
CodeCraft应助chengyi采纳,获得10
14秒前
stuffmatter应助zhgj采纳,获得10
15秒前
Fanny完成签到,获得积分10
15秒前
ephore完成签到,获得积分0
15秒前
16秒前
味子橘给味子橘的求助进行了留言
16秒前
高分求助中
Sustainability in ’Tides Chemistry 2000
Sustainability in ’Tides Chemistry 1500
The ACS Guide to Scholarly Communication 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Handbook of the Mammals of the World – Volume 3: Primates 805
Ethnicities: Media, Health, and Coping 800
Photosynthesis III 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3071500
求助须知:如何正确求助?哪些是违规求助? 2725527
关于积分的说明 7489890
捐赠科研通 2372698
什么是DOI,文献DOI怎么找? 1258220
科研通“疑难数据库(出版商)”最低求助积分说明 610233
版权声明 596916