神经生长因子
原肌球蛋白受体激酶A
血管内皮生长因子
血管内皮生长因子A
神经保护
内分泌学
内科学
视网膜
激酶插入结构域受体
糖尿病性视网膜病变
神经营养素
链脲佐菌素
受体
生物
医学
神经科学
糖尿病
血管内皮生长因子受体
作者
Elena Fico,Pamela Rosso,Viviana Triaca,Marco Segatto,Alessandro Lambìase,Paola Tirassa
出处
期刊:Cells
[MDPI AG]
日期:2022-10-15
卷期号:11 (20): 3246-3246
被引量:7
标识
DOI:10.3390/cells11203246
摘要
Among the factors involved in diabetic retinopathy (DR), nerve growth factor (NGF) and vascular endothelial growth factor A (VEGFA) have been shown to affect both neuronal survival and vascular function, suggesting that their crosstalk might influence DR outcomes. To address this question, the administration of eye drops containing NGF (ed-NGF) to adult Sprague Dawley rats receiving streptozotocin (STZ) intraperitoneal injection was used as an experimental paradigm to investigate NGF modulation of VEGFA and its receptor VEGFR2 expression. We show that ed-NGF treatment prevents the histological and vascular alterations in STZ retina, VEGFR2 expression decreased in GCL and INL, and preserved the co-expression of VEGFR2 and NGF-tropomyosin-related kinase A (TrkA) receptor in retinal ganglion cells (RGCs). The WB analysis confirmed the NGF effect on VEGFR2 expression and activation, and showed a recovery of VEGF isoform dysregulation by suppressing STZ-induced VEGFA121 expression. Reduction in inflammatory and pro-apoptotic intracellular signals were also found in STZ+NGF retina. These findings suggest that ed-NGF administration might favor neuroretina protection, and in turn counteract the vascular impairment by regulating VEGFR2 and/or VEGFA isoform expression during the early stages of the disease. The possibility that an increase in the NGF availability might contribute to the switch from the proangiogenic/apoptotic to the neuroprotective action of VEGF is discussed.
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