胞外聚合物
藻类
恩诺沙星
多糖
分泌物
生物化学
细胞外
内化
化学
绿藻
细胞壁
生物
微生物学
细胞
植物
生物膜
抗生素
细菌
遗传学
环丙沙星
作者
Qilu Cheng,Yangzhi Liu,Li Xu,Jing Ye,Qiang Wang,Hui Lin,Junwei Ma
标识
DOI:10.1016/j.scitotenv.2023.165302
摘要
Algae are susceptible to enrofloxacin (ENR), an antibiotic frequently detected in aquatic environments. However, algal responses, especially the secretion and roles of extracellular polymeric substances (EPS), under ENR exposure remain unknown. This study is the first to elucidate the variation in algal EPS triggered by ENR at both the physiological and molecular levels. The results showed that EPS were significantly (P < 0.05) overproduced along with increased polysaccharide and protein contents in algae exposed to 0.05, 0.5, and 5 mg/L ENR. Secretion of aromatic proteins, especially tryptophan-like substances with more functional groups or aromatic rings, was specifically stimulated. Furthermore, the genes with upregulated expression related to carbon fixation, aromatic protein biosynthesis, and carbohydrate metabolism are direct causes of enhanced EPS secretion. Improved EPS levels increased the cell surface hydrophobicity and provided more adsorption sites for ENR, which strengthened the van der Waals interaction and reduced ENR internalization. The hormesis effects of ENR were alleviated, as illustrated by the less affected cell density, chlorophyll a/b, and carotenoids biosynthesis in algae with EPS. These findings demonstrate the involvement of EPS in algal ENR resistance and promote a deeper understanding of the ecological effects of ENR in aquatic environments.
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