Network pharmacology and molecular docking study for biological pathway detection of cytotoxicity of the yellow jasmine flowers

细胞毒性 细胞凋亡 MTT法 MCF-7型 生物 膜联蛋白 细胞毒性T细胞 活性氧 细胞周期 细胞周期检查点 癌细胞 药理学 化学 传统医学 生物化学 癌症 体外 人体乳房 遗传学 医学
作者
Seham S. El‐Hawary,Marzough Aziz Albalawi,Ayat O. S. Montasser,Shaimaa R. Ahmed,Sumera Qasim,Ali A. Shati,Mohammad Y. Alfaifi,Serag Eldin I. Elbehairi,Omnia F. Hassan,A.A. Sadakah,Fatma Alzahraa Mokhtar
出处
期刊:BMC complementary medicine and therapies [Springer Nature]
卷期号:23 (1) 被引量:1
标识
DOI:10.1186/s12906-023-03987-w
摘要

Abstract Background The yellow jasmine flower ( Jasminum humile L.) is a fragrant plant belonging to the Oleaceae family with promising phytoconstituents and interesting medicinal uses. The purpose of this study was to characterize the plant metabolome to identify the potential bioactive agents with cytotoxic effects and the underlying mechanism of cytotoxic activity. Methods First, HPLC–PDA-MS/MS was used to identify the potential bioactive compounds in the flowers. Furthermore, we assessed the cytotoxic activity of the flower extract against breast cancer (MCF-7) cell line using MTT assay followed by the cell cycle, DNA-flow cytometry, and Annexin V-FITC analyses alongside the effect on reactive oxygen species (ROS). Finally, Network pharmacology followed by a molecular docking study was performed to predict the pathways involved in anti-breast cancer activity. Results HPLC–PDA-MS/MS tentatively identified 33 compounds, mainly secoiridoids. J. humile extract showed a cytotoxic effect on MCF-7 breast cancer cell line with IC 50 value of 9.3 ± 1.2 µg/mL. Studying the apoptotic effect of J. humile extract revealed that it disrupts G2/M phase in the cell cycle, increases the percentage of early and late apoptosis in Annexin V-FTIC, and affects the oxidative stress markers (CAT, SOD, and GSH-R). Network analysis revealed that out of 33 compounds, 24 displayed interaction with 52 human target genes. Relationship between compounds, target genes, and pathways revealed that J. humile exerts its effect on breast cancer by altering, Estrogen signaling pathway, HER2, and EGFR overexpression. To further verify the results of network pharmacology, molecular docking was performed with the five key compounds and the topmost target, EGFR. The results of molecular docking were consistent with those of network pharmacology. Conclusion Our findings suggest that J. humile suppresses breast cancer proliferation and induces cell cycle arrest and apoptosis partly by EGFR signaling pathway, highlighting J. humile as a potential therapeutic candidate against breast cancer.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
徐凤年发布了新的文献求助10
刚刚
rebeccahu应助Kiosta采纳,获得30
1秒前
骐骥过隙完成签到 ,获得积分10
2秒前
2秒前
三三发布了新的文献求助10
3秒前
hbu123应助H先生采纳,获得20
3秒前
隐形曼青应助微微采纳,获得10
3秒前
3秒前
852应助OhahaO采纳,获得10
4秒前
Jasper应助连冷安采纳,获得10
5秒前
犹豫觅翠发布了新的文献求助10
5秒前
万能图书馆应助神可馨采纳,获得10
6秒前
XSY发布了新的文献求助10
6秒前
漂亮幻莲发布了新的文献求助10
7秒前
思源应助研友_842M4n采纳,获得10
7秒前
abbsdan完成签到 ,获得积分10
7秒前
Owen应助哈哈哈采纳,获得10
8秒前
8秒前
9秒前
9秒前
Ava应助火山蜗牛采纳,获得10
9秒前
9秒前
Pursue完成签到,获得积分10
10秒前
今天不学习明天变垃圾完成签到,获得积分10
10秒前
急急急完成签到,获得积分10
10秒前
牵墨完成签到,获得积分10
11秒前
小康完成签到,获得积分10
11秒前
12秒前
呆萌的雁荷完成签到,获得积分10
12秒前
13秒前
款解耦完成签到 ,获得积分10
13秒前
何hyy发布了新的文献求助10
14秒前
14秒前
preeee关注了科研通微信公众号
14秒前
共享精神应助Agao采纳,获得10
15秒前
小康发布了新的文献求助10
15秒前
小栩完成签到 ,获得积分10
15秒前
零九完成签到,获得积分10
15秒前
XSY完成签到,获得积分10
16秒前
greenf发布了新的文献求助10
16秒前
高分求助中
Licensing Deals in Pharmaceuticals 2019-2024 3000
Cognitive Paradigms in Knowledge Organisation 2000
Effect of reactor temperature on FCC yield 2000
Introduction to Spectroscopic Ellipsometry of Thin Film Materials Instrumentation, Data Analysis, and Applications 1200
How Maoism Was Made: Reconstructing China, 1949-1965 800
Medical technology industry in China 600
ANSYS Workbench基础教程与实例详解 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3311876
求助须知:如何正确求助?哪些是违规求助? 2944696
关于积分的说明 8520681
捐赠科研通 2620293
什么是DOI,文献DOI怎么找? 1432756
科研通“疑难数据库(出版商)”最低求助积分说明 664759
邀请新用户注册赠送积分活动 650064