亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Screening and functional verification of the target protein of pedunsaponin A in the killing of Pomacea canaliculata

RNA干扰 蜗牛 生物 福寿螺 去极化 基因 小干扰RNA 细胞生物学 分子生物学 基因表达 核糖核酸 遗传学 生态学 生物物理学
作者
Chunping Yang,Yangyang Zhang,Yue Zhou,Huabao Chen,Tianxing Lv,Liya Luo,Xiaoyan Qiu,Min Zhang,Guangwei Qin,Guoshu Gong
出处
期刊:Ecotoxicology and Environmental Safety [Elsevier]
卷期号:220: 112393-112393 被引量:3
标识
DOI:10.1016/j.ecoenv.2021.112393
摘要

Previous study found that pedunsaponin A (PA) influenced the cytoskeleton of Pomacea canaliculata hemocytes, leading to depolarization and haemocyte destruction and eventually to snail death. In this study, we analysed the changes in protein expression by iTRAQ-mediated proteomics and identified 51 downregulated proteins. Among these, we focused on proteins related to cytoskeletal function and identified neural Wiskott-Aldrich syndrome isoform X1 (PcnWAS). The full-length PcnWAS gene contains 9791 bp and includes an open reading frame of 1401 bp that encodes 735 amino acids with a predicted molecular mass of 49.83 kD. PcnWAS exhibited a relatively distant genetic relationship with known species; the closest homologue is Biomphalaria glabrata (57%). RNA interference (RNAi) was adopted to verify the function of PcnWAS after screening the siRNA sequence with an efficiency of 97%. Interference with the gene expression of PcnWAS did not lead to snail death, but the depolarization level increased, which demonstrated that PcnWAS is an important depolarization-related protein. The results of PA treatment of snails subjected to RNAi proved that interfering with PcnWAS gene expression decreased the molluscicidal activity of PA toward P. canaliculata; snail mortality after RNAi was significantly lower (40%) than that in PA-treated snails without RNAi (54%), while the survival rate and depolarization level in haemocytes were not significant, indicating that PcnWAS is only one of the important target proteins of PA in P. canaliculata. This study lays the foundation for further exploration of the molecular mechanism by which PA kills this harmful snail.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
9秒前
锦鲤完成签到 ,获得积分10
27秒前
52秒前
Later完成签到,获得积分20
59秒前
1分钟前
景泰蓝完成签到,获得积分10
2分钟前
景泰蓝发布了新的文献求助10
2分钟前
2分钟前
3分钟前
3分钟前
鱼块发布了新的文献求助10
3分钟前
FashionBoy应助科研通管家采纳,获得10
3分钟前
赘婿应助鱼块采纳,获得10
3分钟前
一禅完成签到 ,获得积分10
5分钟前
lanbing802完成签到,获得积分10
5分钟前
Jasper应助zjl123采纳,获得10
5分钟前
6分钟前
一杯六一完成签到,获得积分10
6分钟前
7分钟前
zjl123发布了新的文献求助10
7分钟前
gszy1975发布了新的文献求助10
8分钟前
qiuxuan100发布了新的文献求助10
8分钟前
9分钟前
10分钟前
小周完成签到 ,获得积分10
11分钟前
11分钟前
刘刘完成签到 ,获得积分10
11分钟前
kuoping完成签到,获得积分10
12分钟前
王力完成签到 ,获得积分10
12分钟前
StayGolDay完成签到,获得积分10
13分钟前
wanci应助科研通管家采纳,获得10
13分钟前
李健应助科研通管家采纳,获得30
13分钟前
13分钟前
maodeshu应助clement采纳,获得20
14分钟前
纯洁完成签到,获得积分10
14分钟前
14分钟前
草木完成签到,获得积分10
14分钟前
15分钟前
wEric发布了新的文献求助10
15分钟前
15分钟前
高分求助中
Licensing Deals in Pharmaceuticals 2019-2024 3000
Effect of reactor temperature on FCC yield 2000
Very-high-order BVD Schemes Using β-variable THINC Method 1020
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
Mission to Mao: Us Intelligence and the Chinese Communists in World War II 600
The Conscience of the Party: Hu Yaobang, China’s Communist Reformer 600
MATLAB在传热学例题中的应用 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3303289
求助须知:如何正确求助?哪些是违规求助? 2937597
关于积分的说明 8482546
捐赠科研通 2611482
什么是DOI,文献DOI怎么找? 1425949
科研通“疑难数据库(出版商)”最低求助积分说明 662457
邀请新用户注册赠送积分活动 647005