Recombinant expression protein of Type 4 Fimbrial gene (ptfA) of Pasteurella multocida

Abstract The Fimbrial type 4 gene is one of the virulence factor genes associated with bacterial adhesion and colonization factors in Pasteurella multocida . The activity of this gene has a surface covering effect on the host it is ridden on. So that the cell surface in the host is difficult to function. Pasteurella multocida is a microorganism that attacks the upper respiratory tract, especially in buffalo and cattle, causing infection. The aim of this activity was to analyzed the expression and characterization recombinant ptfA for control and elimination of Pasteurella multocida . Gene transformation was carried out using E coli . The induction of gene expression was carried out with IPTG concentrations ranging from 0.1, 0.25, 0.5, 0.75, and 1 mM and incubated at room temperature. The identification analysis was carried out using SDS PAGE showing the 15 KDa gene bands. The 15 kDa recombinant ptfA gene showed the highest expression at a concentration of 0.5 mM of isopropyl thiogalactopyranoside (IPTG).