底漆(化妆品)
寡核苷酸
底漆二聚体
分子生物学
PCR的应用
DNA
硅胶PCR
聚合酶链反应
生物
DNA聚合酶
熔化温度
实时聚合酶链反应
计算生物学
化学
多重聚合酶链反应
生物化学
基因
材料科学
复合材料
有机化学
作者
Yu‐Hsuan Chang,Meng-Wei Wu,Yi‐Ju Chen,Cao-An Vu,Ching-Ya Hong,Wen-Yih Chen
出处
期刊:Methods in molecular biology
日期:2021-11-14
卷期号:: 261-273
被引量:3
标识
DOI:10.1007/978-1-0716-1799-1_18
摘要
This chapter introduces neutralized DNA (nDNA) as a novel design for the primers of PCR and RT-PCR by methylating phosphate groups of some oligonucleotides in their structures. It starts with an introduction of the nDNA which possesses an electrically chimeric neutral backbone as well as the proposed standards in designing nDNA as a novel primer for PCR and RT-PCR , concluded from various experimental results presented afterward. The primary content comprises empirical data from PCR to compare nDNA and unmodified DNA as primers in terms of ability to distinguish and amplify mismatch templates, activities of polymerase enzymes, melting temperature of double-stranded sequences, and the trials and discussions on various modified positions of the nDNA primers. In summary, nDNA exhibited outstanding performance as a primer for PCR and RT-PCR , compared to unmodified DNA, and is expected to be expanded in diverse applications which require enhanced specificity.
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