核酸
分析物
晶体管
检出限
DNA
材料科学
人口
化学
纳米技术
生物化学
色谱法
物理
医学
量子力学
环境卫生
电压
作者
Xuejun Wang,Derong Kong,Mingquan Guo,Liqian Wang,Chenjian Gu,Changhao Dai,Yao Wang,Qunfeng Jiang,Zhaolin Ai,Cong Zhang,Di Qu,Youhua Xie,Zhaoqin Zhu,Yunqi Liu,Dacheng Wei
出处
期刊:Nano Letters
[American Chemical Society]
日期:2021-11-04
卷期号:21 (22): 9450-9457
被引量:49
标识
DOI:10.1021/acs.nanolett.1c02748
摘要
Direct SARS-CoV-2 nucleic acid testing with fast speed and high frequency is crucial for controlling the COVID-19 pandemic. Here, direct testing of SARS-CoV-2 nucleic acid is realized by field-effect transistors (FETs) with an electro-enrichable liquid gate (LG) anchored by tetrahedral DNA nanostructures (TDNs). The applied gate bias electrostatically preconcentrates nucleic acids, while the liquid gate with TDNs provides efficient analyte recognition and signal transduction. The average diagnosis time is ∼80 s, and the limit of detection approaches 1–2 copies in 100 μL of clinical samples without nucleic acid extraction and amplification. As such, TDN-LG FETs solve the dilemma of COVID-19 testing on mass scale that diagnosis accuracy and speed undergo trade-off. In addition, TDN-LG FETs achieve unamplified 10-in-1 pooled nucleic acid testing for the first time, and the results are consistent with PCR. Thus, this technology promises on-site and wide population COVID-19 screening and ensures safe world-reopening.
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