激光捕获显微切割
原位杂交
抗体
分子生物学
生物
免疫组织化学
显微解剖
信使核糖核酸
上皮
病理
基因表达
基因
医学
免疫学
生物化学
作者
Xiaohui Zhu,Cuiying Li,Xin Sun,Yuntao S. Mao,Guohui Li,Xiaoyong Liu,Youhui Zhang,Xiaoyan Qiu
出处
期刊:Applied Immunohistochemistry & Molecular Morphology
日期:2008-04-18
卷期号:16 (3): 232-238
被引量:57
标识
DOI:10.1097/pai.0b013e31814c915a
摘要
In our previous work, we have reported the expression of immunoglobulin (Ig) molecules by numerous epithelial cancer cells and hyperplastic epithelial cells. In the present study, we extended our investigation to study the frequencies of expression of IgG and IgA in some types of oral epithelial tumor cells, and analyzed the oral tumor-derived V regions characteristic of Ig γ chain gene transcripts by immunohistochemistry, in situ hybridization, laser capture microdissection-correlated reverse-transcription polymerase chain reaction, and sequencing. IgG and IgA immunoreactivity was prominent in the cytoplasmic or plasma membrane or secretion of malignant cells, pleomorphic adenoma tumor cells, and some normal glandular epithelial cells or squamous cells adjacent to tumors. More importantly, rearranged Ig gene transcripts were identified in these tumor cells, and in some normal glandular epithelial cells, the V-D-J region sequences revealed that IgG transcripts in 2 tested oral tumors were oligoclonal. These results support that the phenomenon of Ig could also be expressed in oral cavity epithelial tumor cells.
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