During the last decade, immunochromatographic techniques, in particular fluorescence labeled immunochromatography, have become more popular for the determination of low concentrations of analytes. It offers several potential advantages including highly sensitive detection and wide applications in clinical chemistry, bioanalysis, and environmental analysis. Currently, fluorescence labeled immunochromatography is not the exclusive preserve for a few specialists who are well experienced with antigen-antibody analysis, since the recent developments in fluorescence labeled immunochromatography have become widespread, from simple screening application to quantitative analysis of analytes. In this review, we assembled recent advances in the development and applications of fluorescence labeled immunochromatography techniques, considering their potential benefits in the future.