Effect of Helicobacter pylori Infection on the Expression of DNA Mismatch Repair Protein

幽门螺杆菌 微卫星不稳定性 DNA错配修复 胃炎 免疫组织化学 癌症 胃粘膜 萎缩性胃炎 慢性胃炎 染色 内科学 胃肠病学 DNA修复 医学 生物 病理 DNA 基因 微卫星 结直肠癌 遗传学 等位基因
作者
Dong Il Park,Sungha Park,Sang Hoon Kim,Jeong Wook Kim,Yong Kyun Cho,Hong Joo Kim,Chong Il Sohn,Woo Kyu Jeon,Byung Ik Kim,Eun Yoon Cho,Eo‐Jin Kim,Seoung Wan Chae,Jin Hee Sohn,In Kyung Sung,Antonia R. Sepulveda,Jae J. Kim
出处
期刊:Helicobacter [Wiley]
卷期号:10 (3): 179-184 被引量:55
标识
DOI:10.1111/j.1523-5378.2005.00309.x
摘要

Abstract Background. Helicobacer pylori infection is a major gastric cancer risk factor. Deficient DNA mismatch repair (MMR) caused by H. pylori may underlie microsatellite instability (MSI) in the gastric epithelium and may represent a major mechanism of mutation accumulation in the gastric mucosa during the early stages of H. pylori ‐associated gastric carcinogenesis. In this study, we examined the expression of DNA MMR protein (hMLH1 and hMSH2) in patients with chronic H. pylori infection before and after eradication of the infection. Materials and methods. Gastric tissue samples were collected from 60 patients with H. pylori gastritis and peptic ulcer disease before and after eradication of the infection. The DNA MMR protein expression (hMLH1 and hMSH2) was determined by immunohistochemical staining in 60 patients before and after H. pylori eradication. The percentage of epithelial cell nuclei and intensity of staining were then compared in gastric biopsies before and after eradication. Results. The percentage of hMLH1 (76.60 ± 20.27, 84.82 ± 12.73, p = .01) and hMSH2 (82.36 ± 12.86, 88.11 ± 9.27, p < .05) positive epithelial cells significantly increased in 53 patients who became H. pylori ‐negative after eradication therapy. However, the intensity of hMLH1 and hMSH2 staining was not significantly different. In those 7 patients, who did not respond to the eradication therapy and were still H. pylori ‐positive, the percent positivity and intensity of hMLH1 and hMSH2 staining did not change. Conclusions. The expression of DNA MMR proteins increased in the gastric mucosa after H. pylori eradication, indicating that H. pylori gastritis may be associated with a reduced DNA MMR system during infection. The effect of H. pylori infection on MMR protein expression appears to be at least partially reversible after H. pylori eradication. These data suggest that H. pylori gastritis might lead to a deficiency of DNA MMR in gastric epithelium that may increase the risk of mutation accumulation in the gastric mucosa cells during chronic H. pylori infection.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
斯文败类应助霍骁采纳,获得10
刚刚
潘善若发布了新的文献求助10
2秒前
Akim应助阳光采纳,获得10
3秒前
4秒前
20011013完成签到 ,获得积分10
7秒前
8秒前
量子星尘发布了新的文献求助10
9秒前
11秒前
华仔应助机灵飞阳采纳,获得10
12秒前
潘善若发布了新的文献求助10
12秒前
13秒前
陈少华完成签到 ,获得积分10
13秒前
下一秒发布了新的文献求助10
14秒前
杨乃彬完成签到,获得积分10
14秒前
取名叫做利完成签到,获得积分10
15秒前
赘婿应助喻义梅采纳,获得10
16秒前
小二郎应助小门采纳,获得10
17秒前
ll发布了新的文献求助10
20秒前
正直的鸿完成签到,获得积分10
25秒前
26秒前
万能图书馆应助高贵梦露采纳,获得10
27秒前
momo发布了新的文献求助10
29秒前
传奇3应助boltos采纳,获得10
30秒前
30秒前
31秒前
要减肥笑阳完成签到 ,获得积分10
32秒前
全若之发布了新的文献求助10
37秒前
Jasper应助momo采纳,获得10
39秒前
Kasom完成签到 ,获得积分10
46秒前
顺利一德完成签到,获得积分20
47秒前
香蕉觅云应助Afaq采纳,获得10
47秒前
47秒前
47秒前
manman完成签到,获得积分10
48秒前
48秒前
哈哈哈完成签到,获得积分10
48秒前
YamDaamCaa应助科研通管家采纳,获得30
49秒前
49秒前
领导范儿应助科研通管家采纳,获得10
49秒前
香蕉觅云应助科研通管家采纳,获得10
49秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989263
求助须知:如何正确求助?哪些是违规求助? 3531418
关于积分的说明 11253814
捐赠科研通 3270066
什么是DOI,文献DOI怎么找? 1804884
邀请新用户注册赠送积分活动 882084
科研通“疑难数据库(出版商)”最低求助积分说明 809136