穿梭机载体
大肠杆菌
克隆(编程)
质粒
诺卡迪亚
载体(分子生物学)
多克隆站点
选择(遗传算法)
生物
遗传学
克隆载体
微生物学
计算生物学
计算机科学
重组DNA
细菌
人工智能
DNA
基因
程序设计语言
作者
Kazuhiro Chiba,Yasutaka Hoshino,Keiko Ishino,Takahisa Kogure,Yuzuru Mikami,Yoshimasa Uehara,Jun Ishikawa
出处
期刊:PubMed
日期:2007-02-01
卷期号:60 (1): 45-7
被引量:57
摘要
We constructed a pair of Nocardia-Escherichia coli shuttle vectors, pNV18 and pNV19, by combining the mycobacterial plasmid pAL5000 with the E. coli vector pK18 or pK19. These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp.
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