Solid phase extracted-HPLC method for determination of mitoxantrone in cell culture media and intracellular fluid

Objective:To develop a method for determining of mitoxantrone in cell culture media and intracellular fluid by HPLC.Method:Mitoxantrone in cell culture medium and intracellular fluid were extracted by a C_(18)solid phase extraction(SPE).ZORBAX SB-C_(18)(4.6 mm×250 mm,5 μm)was as an analytical column and methanol- 0.02 mol·L~(-1)potassium dihydrogen phosphate(36:64,adjusted pH to 3.0 with orthophosphoric acid).The detec- tion wavelength was 242 nm.Resaults:Mitoxantrone in cell culture media:The concentration range of standard curve was 0.1-4.0 μg·mL~(-1)(r=0.9996,n=5).The average recovery of mitoxantrone was 98.40%,RSD of in- tra-day and inter-day were less than 2%.The limit detection was 0.05 μg·mL~(-1).Mitoxantrone in intracellular fluid:The concentration range of standard curve was 0.5-5.0 μg·mL~(-1)(r=0.9998,n=5).The average recov- ery of mitoxantrone was 91.80%,RSD of intra-day and inter-day were less than 3% and 4%.The limit detec- tion was 0.1 μg·mL~(-1).Conclusion:The method appeares to be simple,accurate and precise.