Isolation, Purification and Bioactivity of Polysaccharides from Cirsium japonicum DC

Medicine and Biopharmaceutical, pp. 1072-1081 (2016) No AccessIsolation, Purification and Bioactivity of Polysaccharides from Cirsium japonicum DCXiao-Jun Chen, Liu Liu, Hua-Xia Qin, Xiu-Ling You, Jiang-Hong Shen and Zhi-Yong LiaoXiao-Jun ChenZhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China, Liu LiuZhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China, Hua-Xia QinZhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China, Xiu-Ling YouZhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China, Jiang-Hong ShenZhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China and Zhi-Yong LiaoZhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, ChinaCorresponding authorhttps://doi.org/10.1142/9789814719810_0137Cited by:0 PreviousNext AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsRecommend to Library ShareShare onFacebookTwitterLinked InRedditEmail Abstract: In this study, polysaccharides Cirsium japonicum DC (CJP) were extracted using both cold and hot water. CJP was then purified by ion exchange resin and chromatography. Results showed that it contained 9.64% of uronic acid and 2.64% of protein. Structural analysis indicated that CJP contained several polysaccharides with different molecular weight of 74.1 kD, 47.0 kD, 5.2 kD and 3.0 kD respectively. Furthermore, HPLC analysis showed that CJP was mainly composed of the following eight monosaccharides: Man (5.50%), Rha (6.73%), GlcA (1.14%), GalA (7.57%), Glc (21.00%), Gal (25.32%), Xyl (3.98%), and Ara (28.75%). Moreover, luciferase report gene assay demonstrated that CJP promoted the activation of both p53 and Rb, two genes playing important role in the development of tumor, indicating a potential antitumor activity for CJP. FiguresReferencesRelatedDetails Medicine and BiopharmaceuticalMetrics History PDF download