色谱法
化学
色谱检测器
漂移管,漂移管
高效液相色谱法
甲酸
校准曲线
梯度洗脱
线性范围
重复性
分析化学(期刊)
质谱法
检出限
作者
Peng Che,Jiushi Liu,Yaodong Qi,Ting-Yan Qiang,Yi-Chen Song,Xueping Wei,Haitao Liu,Bengang Zhang
出处
期刊:China journal of Chinese materia medica
[China Journal of Chinese Materia Medica]
日期:2020-03-01
卷期号:45 (6): 1393-1398
标识
DOI:10.19540/j.cnki.cjcmm.20191223.201
摘要
An UPLC method was established for the direct determination of six major bioactive isosteroidal alkaloids, namely peimisine, imperialine, sipeimine-3-D-glucoside, verticinone, verticine and hupehenine from the bulbus of Fritillaria(Beimu), a commonly used antitussive traditional Chinese medicinal(TCM) herb. An Acquity UPLC~(TM) CSH C_(18) column(2.1 mm×100 mm, 1.7 μm) was used for all analysis. The investigated six compounds were all separated with gradient mobile phase consisting of 0.02% diethylamine-water-methanol at a flow rate of 0.3 mL·min~(-1). The temperature of sample manager was set at 20 ℃. Drift tube temperature was 45 ℃, and spray parameter was 40% with injection volume of 1 μL. Then, the further quality assessment of Beimu was carried out by cluster analysis(CA) and principal component analysis(PCA). The investigated all had good linearity(r≥0.998 9) over the tested ranges. The method is simple, accurate and reproducible, and can be used for determining the content of six major bioactive isosteroidal alkaloids.
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