Low Coverage Whole Genome Sequencing Yields the Complete Mitogenome of Hypselodoris bullocki and Hypselodoris apolegma (Mollusca: Chromodorididae)

Lin, G.; Xiang, P.; Audira, G., and Hsiao, C., 2019. Low coverage whole genome sequencing yields the complete mitogenome of Hypselodoris bullocki and Hypselodoris apolegma (Mollusca: Chromodorididae). In: Hoang, A.T. and Aqeel Ashraf, M. (eds.), Research, Monitoring, and Engineering of Coastal, Port, and Marine Systems. Journal of Coastal Research, Special Issue No. 97, pp. 23–28.In this study, the complete mitogenome sequence of two sea slugs, Hypselodoris bullock and Hypselodoris apolegma (Mollusca: Chromodorididae), has been decoded for the first time by low coverage whole genome sequencing method. The assembled mitogenome, consisting of 14,666 and 14,749 bp, respectively, and has unique 13 protein coding genes (PCGs), 22 transfer RNAs and 2 ribosomal RNAs genes for both species. In Hypselodoris apolegma, one intron sized in 3664 bp is inserted into ND5 gene. The gene order of the mitogenome of Hypselodoris species is almost the same to the other nudibranch mitogenomes, while the position of tRNA-Ser and ND4 gene moved to between tRNA-Cys and tRNA-Gln. In addition, we found the nucleotide diversity is about four times higher in Hypselodoris when Chromodoris species are compared. The complete mitogenome of Hypselodoris bullock and Hypselodoris apolegma provides essential and important DNA molecular data for phylogenetic and evolutionary analysis for Chromodorididae.