间充质干细胞
骨髓
细胞生物学
锶
蛋白质数据库
化学
碱性磷酸酶
间质细胞
雷奈酸锶
细胞生长
生物
免疫学
癌症研究
内分泌学
生物化学
骨质疏松症
酶
有机化学
作者
Yizhou Huang,Chengguang Wu,Huiqi Xie,Zhaoyang Li,Antonietta Rosa Silini,Ornella Parolini,Yi Wu,Li Deng,Yong‐Can Huang
摘要
The osteogenic potential of mesenchymal stromal cells (MSCs) varies among different tissue sources. Strontium enhances the osteogenic differentiation of bone marrow-derived MSCs (BM-MSCs), but whether it exerts similar effects on placental decidual basalis-derived MSCs (PDB-MSCs) remains unknown. Here, we compared the influence of strontium on the proliferation and osteogenic differentiation of human PDB- and BM-MSCs in vitro . We found that 1 mM and 10 mM strontium, but not 0.1 mM strontium, evidently promoted the proliferation of human PDB- and BM-MSCs. These doses of strontium showed a comparable alkaline phosphatase activity in both cell types, but their osteogenic gene expressions were promoted in a dose-dependent manner. Strontium at doses of 0.1 mM and 1 mM elevated several osteogenic gene expressions of PDB-MSCs, but not those of BM-MSCs at an early stage. Nevertheless, they failed to enhance the mineralization of either cell type. By contrast, 10 mM strontium facilitated the osteogenic gene expression as well as the mineralization of human PDB- and BM-MSCs. Collectively, this study demonstrated that human PDB- and BM-MSCs shared a great similarity in response to strontium, which promoted their proliferation and osteogenic differentiation in a dose-dependent manner.
科研通智能强力驱动
Strongly Powered by AbleSci AI