化学
色谱法
原儿茶酸
芦丁
槲皮素
没食子酸
儿茶素
抗氧化剂
类黄酮
高效液相色谱法
绿原酸
质谱法
多酚
山奈酚
串联质谱法
生物化学
作者
Yayun Zhao,Honglin Lu,Qiang Wang,Hailiang Liu,Haitao Shen,Wenbin Xu,Juan Ge,Dajun He
标识
DOI:10.1002/jssc.202000962
摘要
Abstract Ribes meyeri leaves are used as traditional Kazakh medicine in China. However, no study on the characterization of the phenolic compounds in R. meyeri leaves has been reported, resulting in the lack of quality control measures and poor standardization. This study was conducted to identify the phenolic compounds in R. meyeri leaves and evaluate their antioxidant and antidiabetic activities. A total of 77 phenolics were tentatively identified by liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry. Ultra‐high performance liquid chromatography coupled with triple quadrupole mass spectrometry was applied to simultaneously quantify 12 phenolics in R. meyeri leaves. Rutin, epigallocatechin, isoquercitrin, epicatechin, protocatechuic acid, and kaempferol‐3‐ O ‐rutinoside were abundant in the R. meyeri leaves. The methanol extract and four different extracts enhanced the glucose uptake in 3T3‐L1 adipocytes. The ethyl acetate extracts showed a total phenolic content of 966.89 ± 3.59 mg gallic acid equivalents/g, a total flavonoid content of 263.58 ± 17.09 mg catechin equivalents/g, and good protein‐tyrosine phosphatase‐1B inhibitory activities (IC 50 : 0.60 ± 0.03 μg/mL). To our knowledge, this work is the first to identify and quantify the major phenolics in R. meyeri leaves.
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