Plant stem-cell organization and differentiation at single-cell resolution

Significance Plants possess the remarkable ability to grow and produce new organs throughout their lifespan, owing to the activities of persistent populations of pluripotent stem cells within their meristematic tips. Here we isolated individual cells from the microscopic shoot apical meristem (SAM) of maize and provide single-cell transcriptomic analysis of a plant shoot meristem. This study enabled an unbiased analysis of the developmental genetic organization of the maize shoot apex and uncovered evolutionarily divergent and conserved signatures of SAM homeostasis. The fine-scale resolution of single-cell analysis was used to reconstruct the process of shoot cell differentiation, whereby stem cells acquire diverse and distinct cell fates over developmental time in wild-type and mutant maize seedlings.