生物
DNA条形码
进化生物学
细胞色素c氧化酶亚单位Ⅰ
生物多样性
线粒体DNA
DNA测序
计算生物学
条形码
生态学
基因
遗传学
计算机科学
操作系统
作者
Carmelo Andújar,Paula Arribas,Douglas W. Yu,Alfried P. Vogler,Brent C. Emerson
摘要
Metabarcoding of complex metazoan communities is increasingly being used to measure biodiversity in terrestrial, freshwater and marine ecosystems, revolutionizing our ability to observe patterns and infer processes regarding the origin and conservation of biodiversity. A fundamentally important question is which genetic marker to amplify, and although the mitochondrial cytochrome oxidase subunit I (COI) gene is one of the more widely used markers in metabarcoding for the Metazoa, doubts have recently been raised about its suitability. We argue that (a) the extensive coverage of reference sequence databases for COI; (b) the variation it presents; (c) the comparative advantages for denoising protein-coding genes; and (d) recent advances in DNA sequencing protocols argue in favour of standardizing for the use of COI for metazoan community samples. We also highlight where research efforts should focus to maximize the utility of metabarcoding.
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