光动力疗法
光敏剂
体内
化学发光
单线态氧
肿瘤微环境
化学
癌症研究
生物物理学
光化学
纳米技术
纳米反应器
材料科学
纳米颗粒
有机化学
氧气
医学
肿瘤细胞
生物技术
生物
作者
Ming Wu,Lingjie Wu,Jiong Li,Da Zhang,Shanyou Lan,Xiaolong Zhang,Xinyi Lin,Gang Liu,Xiaolong Liu,Jingfeng Liu
出处
期刊:Theranostics
[Ivyspring International Publisher]
日期:2019-01-01
卷期号:9 (1): 20-33
被引量:57
摘要
The low tissue penetration depth of external excitation light severely hinders the sensitivity of fluorescence imaging (FL) and the efficacy of photodynamic therapy (PDT) in vivo; thus, rational theranostic platforms that overcome the light penetration depth limit are urgently needed.To overcome this crucial problem, we designed a self-luminescing nanosystem (denoted POCL) with near-infrared (NIR) light emission and singlet oxygen ( 1 O2) generation abilities utilizing an intraparticle relayed resonance energy transfer strategy.Methods: Bis[3,4, phenyl] oxalate (CPPO) as a chemical energy source with high reactivity toward H2O2, poly[(9,9'-dioctyl-2,7-divinylene-fluorenylene)-alt-2-methoxy-5-(2-ethyl-hexyloxy)-1,4-phenylene] (PFPV) as a highly efficient chemiluminescence converter, and tetraphenylporphyrin (TPP) as a photosensitizer with NIR emission and 1 O2 generation abilities were coencapsulated by self-assembly with poly(ethyleneglycol)-co-poly(caprolactone) (PEG-PCL) and folate-PEG-cholesterol to form the POCL nanoreactor, with folate as the targeting group.A series of in vitro and in vivo analyses, including physical and chemical characterizations, tumor targeting ability, tumor microenvironment activated imaging and photodynamic therapy, as well as biosafety, were systematically investigated to characterize the POCL. Results:The POCL displayed excellent NIR luminescence and 1 O2 generation abilities in response to H2O2.Therefore, it could serve as a specific H2O2 probe to identify tumors through chemiluminescence imaging and as a chemiluminescence-driven PDT agent for inducing tumor cell apoptosis to inhibit tumor growth due to the abnormal overproduction of H2O2 in the tumor microenvironment.Moreover, the folate ligand on the POCL surface can further improve the accumulation at the tumor site via a receptor-mediated mechanism, thus enhancing tumor imaging and the therapeutic effects both in vitro and in vivo but without any observable systemic toxicity. Conclusion:The nanosystem reported here might serve as a targeted, smart, precise, and noninvasive strategy triggered by the tumor microenvironment rather than by an outside light source for cancer NIR imaging and PDT treatment without limitations on penetration depth.
科研通智能强力驱动
Strongly Powered by AbleSci AI