Transformation of <i>GBSS I</i> Gene Driven with Barley Endosperm-specific Promoter into Maize Inbred Line Mediated by <i>Agrobacterium</i>

In present study, We made a plant expression construct that ligated GBSS I  cloned from common wheat and endosperm specific promoter HorD from barley into pCAMBIA3301 by using the two bridge vector pGM-T-HorD and pGM-T-GBSSI , then the construct named pHorD-GBSSI was transformed into EHA105. Somatic embryogenesis of inbred line Y423 was used to be transformed by Agrobacterium tumefaciens mediation. Regenerated plants were harvested to be genetically detected and total of 35 palnrts were positive by PCR accreditation, accounting for 15% . We thought this study might provide a molecular way to improve the quality of maize starch.