GPX2 stabilized by PCBP2 induces autophagy to protect Het-1A esophageal cells from apoptosis and inflammation

生物 谷胱甘肽过氧化物酶 炎症 癌症研究 分子生物学 内科学 内分泌学 免疫学 超氧化物歧化酶 氧化应激 医学
作者
Shaoqiu Wu,Xiangkang Yin,Ning Xia,Peng Zhang,Bingyan Liu,Li Weng,Mingyi Shang
出处
期刊:Cellular Signalling [Elsevier BV]
卷期号:97: 110397-110397 被引量:3
标识
DOI:10.1016/j.cellsig.2022.110397
摘要

The implantation of esophageal stent loaded with iodine-125 ( 125 I) is one of the critical approaches for the treatment of advanced esophageal cancer, but one common complication after stent implantation is benign hyperplasia associated restenosis. The aim of the current study is to understand the role of glutathione peroxidase 2 (GPX2), a key member of the GPX family of antioxidant enzymes. Initially, we detected the increased levels of GPX2 and GPX1 in esophageal benign hyperplasia tissues. Further, we evaluated the effects of GPX2 in response to H 2 O 2 induced apoptosis and LPS induced inflammation. We observed that GPX2 expression was also significantly increased in Het-1A. Silencing of GPX2 significantly increased H 2 O 2 -induced apoptosis and LPS-induced inflammation, but GPX2 overexpression did the opposite, suggesting a protective effect by GPX2. In addition, we identified that the protective effect of GPX2 is through the activation of autophagy, and PCBP2, a poly (rC) binding protein, binds to and stabilizes GPX2 mRNA. These findings reveal a role of GPX2 in esophageal hyperplasia, allowing for a better understanding of the underlying molecular mechanism, which could lead to identification of potential treatment for clinical benign restenosis following stent implantation with 125 I. • GPX2 expression was significantly increased in Het-1A esophageal cells. • Knockdown GPX2 significantly increased H 2 O 2 -induced apoptosis and LPS-induced inflammation. • The protective effect of GPX2 is through the activation of autophagy. • PCBP2, a poly (rC) binding protein, binds to and stabilizes GPX2 mRNA.
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