Antibiotics in fish caught from ice-sealed waters: Spatial and species variations, tissue distribution, bioaccumulation, and human health risk

Antibiotics are increasingly detected in fish caught in ice-free waters, but information on fish caught in ice-sealed waters is insufficient. The concentrations of 23 antibiotics in the gills, muscles, kidneys, livers, biles, and brains of Cyprinus carpio and Hypophthalmichthys nobilis caught during winter fish-hunting activities in Chagan Lake, Haernao Reservoir, and Shitoukoumen Reservoir were systematically studied to ascertain the variations among fish species and fishing regions, tissue distribution, and bioaccumulation, as well as the potential risk to humans via the consumption of contaminated fish. The results indicated that the individual antibiotic concentration in tissues ranged from undetectable to 35.0 ng/g ww. The total antibiotic concentration in fish muscles from Shitoukoumen Reservoir was lower than that from Chagan Lake and Haernao Reservoir, but showed no significant difference between Cyprinus carpio and Hypophthalmichthys nobilis. Chloramphenicols had a high proportion in most fish tissues ranging from 28.3% to 44.0%, and the antibiotics were mainly distributed in the livers with a total concentration of 54.8 ± 9.9 ng/g ww. The mean values of bioaccumulation factors (BAF) of antibiotics in tissues ranged from 79.4 to 1000 L/kg, with the higher values found in the fish livers. The hazard quotient and hazard index value of antibiotics in the muscles of fish from ice-sealed were less than 1, indicating a negligible risk to human health via the consumption of fish muscles. This study revealed that the total antibiotic concentration in muscles showed spatial variations but not fish species-dependence. The antibiotics mainly accumulated in the livers. In addition, the target antibiotic concentrations in the muscles of fish from ice-sealed waters met the safe for consumption criteria.