Molecular dosimetry in rat urine of aflatoxin-N7-guanine and other aflatoxin metabolites by multiple monoclonal antibody affinity chromatography and immunoaffinity/high performance liquid chromatography.

黄曲霉毒素 鸟嘌呤 亲和层析 色谱法 化学 尿 单克隆抗体 高效液相色谱法 生物化学 抗体 生物 食品科学 免疫学 核苷酸 基因
作者
John D. Groopman,Julia A. Hasler,Laura J. Trudel,Anthony Pikul,Paul R. Donahue,Gerald N. Wogan
出处
期刊:PubMed 卷期号:52 (2): 267-74 被引量:79
链接
标识
摘要

The development of molecular dosimetry methods will simplify the identification of people at high risk for cancer. A combined monoclonal antibody immunoaffinity chromatography/high performance liquid chromatography method has been devised to isolate and quantify aflatoxin-DNA adducts and other metabolites in rat urine samples. We report the production of 11 different monoclonal antibodies recognizing aflatoxin B1, aflatoxin Q1, aflatoxin G1, aflatoxicol, and aflatoxin M1 and the application of these antibodies to a multiple monoclonal antibody affinity chromatography technique. Using the multiple monoclonal antibody affinity column with rat urines obtained from dosed animals, between 90 and 95% of total aflatoxin metabolites can be bound to the column and isolated. Analytical immunoaffinity chromatography/high performance liquid chromatography analysis of these isolated aflatoxins reveals that more than 55% of the aflatoxins in rat urine are aflatoxin-dihydrodiol, aflatoxin-N7-guanine, aflatoxin Q1, aflatoxin M1, aflatoxin P1, and aflatoxin B1, accounting for 1.5, 9.6, 1.8, 34.5, 8.0, and 1.0% of the total aflatoxins, respectively. Further, a perchloric acid digestion of the aflatoxin-N7-guanine peak was used to confirm its identity by its conversion to guanine. The measurement of aflatoxin-N7-guanine excretion in rat urine was examined to assess its utility as a marker of DNA adduct formation in the liver, and a dose-dependent excretion in urine was found with a correlation coefficient of 0.99. A comparison of the dose-dependent residual levels of aflatoxin binding to liver DNA with the amount of aflatoxin-N7-guanine excreted in urine showed a correlation coefficient of 0.98. Besides the nucleic acid adduct excretion data, aflatoxin M1 and aflatoxin P1 were evaluated as molecular dosimeters in the urine. Aflatoxin M1 was found to be an excellent marker, whereas no linear relationship between dose and aflatoxin P1 excretion in urine was found.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
完美芫发布了新的文献求助10
1秒前
WD发布了新的文献求助10
1秒前
2秒前
像风一样发布了新的文献求助20
2秒前
3秒前
跳不起来的大神完成签到 ,获得积分10
4秒前
xiong_mandy完成签到 ,获得积分20
4秒前
5秒前
5秒前
科研通AI2S应助yuchangkun采纳,获得10
6秒前
6秒前
积极的邴完成签到 ,获得积分10
6秒前
6秒前
6秒前
称心背包发布了新的文献求助10
6秒前
7秒前
义气代梅完成签到,获得积分20
7秒前
kongbaisaigao发布了新的文献求助10
7秒前
科目三应助静谧180采纳,获得10
7秒前
科研通AI2S应助文静的翠安采纳,获得10
7秒前
Hello应助科研小菜采纳,获得10
7秒前
斯文败类应助笑点低蜜蜂采纳,获得10
8秒前
水煮蛋完成签到,获得积分10
8秒前
9秒前
10秒前
赵小漂亮发布了新的文献求助10
10秒前
yyyyyy完成签到,获得积分20
11秒前
科研Cat完成签到,获得积分10
11秒前
浓雾发布了新的文献求助10
11秒前
酷波er应助受伤的小松鼠采纳,获得10
11秒前
GKT发布了新的文献求助10
12秒前
爱丽丝敏发布了新的文献求助10
12秒前
12秒前
若邻发布了新的文献求助30
12秒前
Orange应助Eva采纳,获得10
13秒前
13秒前
葡萄关注了科研通微信公众号
14秒前
JamesPei应助77采纳,获得10
14秒前
飒飒玛完成签到,获得积分10
14秒前
英俊的铭应助kilig采纳,获得10
15秒前
高分求助中
Sustainability in Tides Chemistry 1500
Handbook of the Mammals of the World – Volume 3: Primates 805
Ethnicities: Media, Health, and Coping 800
Gerard de Lairesse : an artist between stage and studio 500
Digging and Dealing in Eighteenth-Century Rome 500
Queer Politics in Times of New Authoritarianisms: Popular Culture in South Asia 500
Manual of Sewer Condition Classification 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3069006
求助须知:如何正确求助?哪些是违规求助? 2722831
关于积分的说明 7479538
捐赠科研通 2369753
什么是DOI,文献DOI怎么找? 1256697
科研通“疑难数据库(出版商)”最低求助积分说明 609645
版权声明 596839