Measurement of Adhesion Under Flow Conditions

This unit describes the analysis of dynamic cell adhesion using a flow chamber assay. The flow chamber enables the researcher to reconstruct cell systems in the presence of shear stress to assay adhesion under well&defined forces. These assays are most commonly used to study leukocyte adhesion, either to cultured endothelial cell monolayers or to purified substrates, simulating physiological interactions of leukocytes with endothelial cells. This assay can be also be used to characterize transient adhesive events or adhesion strengthening even for cells that do not normally experience shear stress, because contact time between cells and substrates and anti&adhesive forces can be closely regulated by stopping and starting the flow. Flow chamber assays are also useful for measuring bacterial adhesion under flow.