DNA-Directed Assembly of Bienzymic Complexes from In Vivo Biotinylated NAD(P)H:FMN Oxidoreductase and Luciferase

Spatially controlled assembly of enzyme conjugates can be applied to signal amplification systems for biosensors, or synthesis of novel catalysts for enzyme process technology. Artificial bienzymic systems comprised of two enzymes, NAD(P)H-FMN oxidoreductase and luciferase (represented as blue and orange spheres, respectively, in the schematic representation) were assembled by means of DNA hybridization and streptavidin–biotin interaction.