经颅直流电刺激
医学
神经可塑性
初级运动皮层
脑源性神经营养因子
运动皮层
神经科学
神经营养因子
前肢
原肌球蛋白受体激酶B
冲程(发动机)
中风恢复
刺激
内科学
受体
心理学
解剖
物理疗法
工程类
机械工程
康复
作者
Valentina Longo,Saviana Antonella Barbati,Agnese Re,Fabiola Paciello,Maria Bolla,Marco Rinaudo,Francesca Miraglia,Francesca Alù,Martina Gaia Di Donna,Fabrizio Vecchio,Paolo Maria Rossini,Maria Vittoria Podda,Claudio Grassi
出处
期刊:Stroke
[Ovid Technologies (Wolters Kluwer)]
日期:2022-05-01
卷期号:53 (5): 1746-1758
被引量:31
标识
DOI:10.1161/strokeaha.121.034200
摘要
More effective strategies are needed to promote poststroke functional recovery. Here, we evaluated the impact of bihemispheric transcranial direct current stimulation (tDCS) on forelimb motor function recovery and the underlying mechanisms in mice subjected to focal ischemia of the motor cortex.Photothrombotic stroke was induced in the forelimb brain motor area, and tDCS was applied once per day for 3 consecutive days, starting 72 hours after stroke. Grid-walking, single pellet reaching, and grip strength tests were conducted to assess motor function. Local field potentials were recorded to evaluate brain connectivity. Western immunoblotting, ELISA, quantitative real-time polymerase chain reaction, and Golgi-Cox staining were used to uncover tDCS-mediated stroke recovery mechanisms.Among our results, tDCS increased the rate of motor recovery, anticipating it at the early subacute stage. In this window, tDCS enhanced BDNF (brain-derived neurotrophic factor) expression and dendritic spine density in the peri-infarct motor cortex, along with increasing functional connectivity between motor and somatosensory cortices. Treatment with the BDNF TrkB (tropomyosin-related tyrosine kinase B) receptor inhibitor, ANA-12, prevented tDCS effects on motor recovery and connectivity as well as the increase of spine density, pERK (phosphorylated extracellular signal-regulated kinase), pCaMKII (phosphorylated calcium/calmodulin-dependent protein kinase II), pMEF (phosphorylated myocyte-enhancer factor), and PSD (postsynaptic density)-95. The tDCS-promoted rescue was paralleled by enhanced plasma BDNF level, suggesting its potential role as circulating prognostic biomarker.The rate of motor recovery is accelerated by tDCS applied in the subacute phase of stroke. Anticipation of motor recovery via vicariate pathways or neural reserve recruitment would potentially enhance the efficacy of standard treatments, such as physical therapy, which is often delayed to a later stage when plastic responses are progressively lower.
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