Integrated single-cell sequencing and histopathological analyses reveal diverse injury and repair responses in a participant with acute kidney injury: a clinical-molecular-pathologic correlation

Acute kidney injury (AKI) induces a broad range of clinically significant perturbations in kidney morphology and function. Insights into the molecular and cellular basis governing a clinical-pathologic course are limited by the availability of molecular maps of healthy and injured kidney cells. The Kidney Precision Medicine Project (KPMP) and the Human Biomolecular Atlas Project (HuBMAP) have generated a comprehensive molecular atlas of the kidney from more than 400,000 cells that define healthy and injured cell types and states in AKI and chronic kidney disease. 1 de Boer I.H. Alpers C.E. Azeloglu E.U. et al. Rationale and design of the Kidney Precision Medicine Project. Kidney Int. 2021; 99: 498-510 Abstract Full Text Full Text PDF PubMed Scopus (63) Google Scholar ,2 Tuttle K.R. Bebiak J. Brown K. et al. Patient perspectives and involvement in precision medicine research. Kidney Int. 2021; 99: 511-514 Abstract Full Text Full Text PDF PubMed Scopus (2) Google Scholar Using the atlas as a resource to perform deep interrogation of a biopsy from a patient participant with AKI, we applied single-cell analysis and detailed morphologic and pathway analysis to gain molecular insights into cellular diversity, injury states, and outcome. In this issueKidney InternationalVol. 101Issue 6PreviewIn this issue of Kidney International, we are pleased to introduce a new article series called Next Generation Clinical Pathologic Conference (CPC). These articles will merge the traditional CPC with molecular medicine for a fresh approach to decipher mechanisms of kidney disease. In cooperation with the National Institutes of Health–sponsored Kidney Precision Medicine Project, biopsies from patients with chronic kidney disease (CKD) or acute kidney injury (AKI) that have been interrogated with cutting-edge molecular technologies will be reviewed and correlated with clinical and histopathologic findings. Full-Text PDF