Exploring the resistome, virulome, mobilome and microbiome along pork production chain using metagenomics

抵抗性 流动遗传元素 生物 基因组 微生物群 质粒 毒力 抗生素耐药性 微生物学 基因 遗传学 抗生素
作者
Lili Li,Yawen Xiao,Chong Wang,Rikke Heidemann Olsen,Hecheng Meng,Lei Shi
出处
期刊:International Journal of Food Microbiology [Elsevier BV]
卷期号:371: 109674-109674 被引量:6
标识
DOI:10.1016/j.ijfoodmicro.2022.109674
摘要

In order to understand and minimize microbial contaminants spread from animal to raw pork products, we explored the diversity of antibiotic resistance genes (ARGs), virulence factors (VFs), mobile genetic elements (MGEs) and the bacterial community composition in feces of pigs, processing areas as well as the end pork products in a large-scale pig slaughterhouse in China using metagenomics. The abundance and diversity of microbial community was higher in arrival and slaughtering room area and decreased sharply in the end pork products. Furthermore, the relative abundance of some clinically relevant pathogens and opportunity pathogens were greater in the end pork products and cutter samples. We identified 1412 subtypes of ARGs related to 30 antibiotic classes, in which ARGs related to multidrug resistance and β-lactamase were dominant. Resistance determinants to clinically critical important antibiotics, including sequences related to mcr, optrA, poxtA, tetX and β-lactamase genes (i.e. blaOXA, blaVIM, blaIMP, blaGES, blaNDM, blaKPC and blaSME) were detected. More than 42 general virulence features, mainly adherence, secretion system, iron uptake, toxin, antiphagocytosis and immune evasion, were identified. A total of 1922 types of MGEs, mainly plasmids were observed. Most of the ARGs are predicted to be associated with MGEs. The prevalence of ARGs, VFs and MGEs decreased over subsequential processing steps. Most of the remaining ARGs, VFs and MGEs in end pork products were also present on other samples, indicating the flow of these genes through the production line. These results broaden our understanding of the global ARGs, VFs and MGEs diversity along the pork production chain, with the suggestion of implementing improved control measures to reduce the risk of spread of pathogenic bacteria and their associated resistome, virulome and mobilome from animal to the food chain and the surrounding environment.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
josie完成签到 ,获得积分10
3秒前
llll完成签到 ,获得积分10
3秒前
量子星尘发布了新的文献求助10
3秒前
韭菜发布了新的文献求助10
3秒前
外向的斑马完成签到 ,获得积分10
4秒前
村长热爱美丽完成签到 ,获得积分10
6秒前
尹尹关注了科研通微信公众号
8秒前
呆呆完成签到 ,获得积分10
9秒前
xianyaoz完成签到 ,获得积分0
16秒前
杨远杰完成签到,获得积分10
17秒前
蓝桉完成签到 ,获得积分10
17秒前
JuliaWang完成签到 ,获得积分10
24秒前
无限的含羞草完成签到,获得积分10
25秒前
八二力完成签到 ,获得积分10
29秒前
韭菜发布了新的文献求助10
32秒前
情怀应助科研通管家采纳,获得30
35秒前
water应助科研通管家采纳,获得10
35秒前
JamesPei应助科研通管家采纳,获得10
35秒前
2012csc完成签到 ,获得积分0
37秒前
风清扬应助韭菜采纳,获得10
38秒前
WSY完成签到 ,获得积分10
39秒前
虞无声发布了新的文献求助10
40秒前
执着新蕾完成签到,获得积分10
42秒前
Vivian完成签到 ,获得积分10
45秒前
666完成签到 ,获得积分10
47秒前
52秒前
量子星尘发布了新的文献求助10
55秒前
蔡从安完成签到,获得积分10
55秒前
奥雷里亚诺完成签到 ,获得积分10
55秒前
不呆完成签到 ,获得积分10
56秒前
Cheung2121发布了新的文献求助30
56秒前
画龙完成签到,获得积分10
57秒前
韭菜完成签到,获得积分20
58秒前
Owen应助Cheung2121采纳,获得10
1分钟前
爱学习的小李完成签到 ,获得积分10
1分钟前
若水完成签到 ,获得积分10
1分钟前
SYLH应助tian采纳,获得10
1分钟前
脑洞疼应助tian采纳,获得10
1分钟前
Ava应助tian采纳,获得10
1分钟前
领导范儿应助tian采纳,获得10
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038112
求助须知:如何正确求助?哪些是违规求助? 3575788
关于积分的说明 11373801
捐赠科研通 3305604
什么是DOI,文献DOI怎么找? 1819255
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022