聚己内酯
再生(生物学)
坐骨神经
材料科学
静电纺丝
神经导管
脑源性神经营养因子
生物医学工程
神经营养因子
神经再生
组织工程
碳纳米管
神经营养素
化学
纳米技术
解剖
细胞生物学
复合材料
聚合物
医学
受体
生物化学
生物
作者
Wei Pi,Yanling Zhang,Longfei Li,C. Li,Meng Zhang,Zhang We,Qing Cai,Peixun Zhang
出处
期刊:Biofabrication
[IOP Publishing]
日期:2022-04-20
卷期号:14 (3): 035006-035006
被引量:29
标识
DOI:10.1088/1758-5090/ac57a6
摘要
Abstract Carbon nanotubes (CNTs) have attracted increasing attention in the field of peripheral nerve tissue engineering due to their unique structural and physical characteristics. In this study, a novel type of aligned conductive scaffolds composed of polycaprolactone (PCL) and CNTs were fabricated via electrospinning. Utilizing mussel-inspired polydopamine (PDA) surface modification, brain-derived neurotrophic factor (BDNF) was loaded onto PCL/CNT fibrous scaffolds to obtain PCL/CNT-PDA-BDNF fibrous scaffolds capable of the sustained release of BDNF over 28 d. Schwann cells were cultured on these scaffolds, and the effect of the scaffolds on peripheral nerve regeneration in vitro was assessed by studying cell proliferation, morphology and the expressions of myelination-related genes S100, P0 and myelin basic protein. Furthermore, the effect of these scaffolds on peripheral nerve regeneration in vivo was investigated using a 10 mm rat sciatic nerve defect model. Both the in vitro and in vivo results indicate that PCL/CNT-PDA-BDNF fibrous scaffolds effectively promote sciatic nerve regeneration and functional recovery. Therefore, PCL/CNT-PDA-BDNF fibrous scaffolds have great potential for peripheral nerve restoration.
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