Sirt3 negatively regulates Glut4 in skeletal muscle insulin resistance in old male offspring rats fed with maternal high fat diet

内科学 内分泌学 后代 胰岛素抵抗 过剩4 骨骼肌 生物 胰岛素 糖耐量试验 怀孕 医学 遗传学
作者
Pengjie Zhang,Yanping Liu,Dan Zhu,Xionghui Chen,Yueming Zhang,Xiuwen Zhou,Qin Huang,Min Li,Youguo Chen,Miao Sun
出处
期刊:Journal of Nutritional Biochemistry [Elsevier BV]
卷期号:104: 108970-108970 被引量:2
标识
DOI:10.1016/j.jnutbio.2022.108970
摘要

High fat (HF) diet intake during pregnancy is linked to insulin resistance (IR) in offspring, but the molecular mechanism still needs to be clarified. In the present study, pregnant rats were fed either HF diet (45% energy from fat) or normal control (CON) diet (10% energy from fat) during gestation from day 5-21. Experiments were performed in 20 months old male offspring rats. The results showed that the fasting insulin levels of old male offspring in HF increased compared with CON. However, the fasting glucose levels was unchanged. Besides, the glucose tolerance tests (GTTs) and insulin tolerance tests (ITTs) were impaired, and the AUC of GTT in HF and CON was 44.62±5.12, 33.58±3.98, with significant differences (P<.05). Furthermore, the AUC of ITT in HF and CON was 16.62±2.07 and 12.44±2.28, which showed an obvious difference (P<.05). Additionally, Sirt3 transcript and protein expressions were found up-regulated in both of the HF old male offspring skeletal muscle (1.42-fold) (P<.05) and palmitic acid (PA) treated L6 myotubes (1.32-fold) (P<.05), accompanied with increase in nicotinamide adenine dinucleotide (NAD+) in HF old male offspring skeletal muscle (P<.05). What's more, Glut4 transcript and protein expressions were found decreased significantly in both of the HF old male offspring skeletal muscle (0.45-fold) (P<.01) and PA treated L6 myotubes (0.49-fold) (P<.01). Transfection of Sirt3 gene in differentiated L6 myocytes indicated that Sirt3 negatively regulated Glut4 in L6 cells. However, AcK-103 protein, an indicator of acetylation, was found decreased in HF old male offspring skeletal muscle and L6 myotubes as a result of up-regulation of Sirt3 (P<.05). Thus, our findings suggest that HF diet intake during pregnancy induced IR in the skeletal muscle of old male offspring rats, probably occurred through Sirt3-Glut4 retrograde regulation, providing novel information concerning the molecular mechanism of IR in old male offspring rats.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
yao完成签到,获得积分10
刚刚
常芹发布了新的文献求助10
1秒前
ED应助科研路漫漫采纳,获得10
1秒前
2秒前
布鲁克完成签到,获得积分10
3秒前
3秒前
量子星尘发布了新的文献求助10
4秒前
今后应助朴实的绣连采纳,获得30
4秒前
<小天才>发布了新的文献求助10
6秒前
6秒前
7秒前
Smy完成签到 ,获得积分10
7秒前
在水一方应助梁晓雯采纳,获得10
8秒前
Yy123发布了新的文献求助10
8秒前
tao发布了新的文献求助10
8秒前
3237924531完成签到,获得积分10
8秒前
健忘小霜完成签到,获得积分10
9秒前
10秒前
scholar完成签到,获得积分10
11秒前
wei发布了新的文献求助10
11秒前
鳗鱼灵阳完成签到,获得积分20
12秒前
12秒前
13秒前
无情的聋五完成签到 ,获得积分10
13秒前
Owen应助QQiang6采纳,获得10
14秒前
14秒前
SciGPT应助wudizhuzhu233采纳,获得10
14秒前
夏天应助wudizhuzhu233采纳,获得150
14秒前
不宁不令发布了新的文献求助20
14秒前
戴佳伟彩笔完成签到,获得积分10
15秒前
15秒前
英俊的绮波完成签到,获得积分10
15秒前
15秒前
15秒前
Ava应助Yy123采纳,获得10
15秒前
17秒前
科目三应助科研小贩采纳,获得10
17秒前
lll发布了新的文献求助10
19秒前
mingming发布了新的文献求助10
20秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988838
求助须知:如何正确求助?哪些是违规求助? 3531250
关于积分的说明 11252914
捐赠科研通 3269838
什么是DOI,文献DOI怎么找? 1804820
邀请新用户注册赠送积分活动 881943
科研通“疑难数据库(出版商)”最低求助积分说明 809028