Z-scheme Bi2O3/CuBi2O4 heterojunction enabled sensitive photoelectrochemical detection of aflatoxin B1 for health care, the environment, and food

Designing a photoelectrochemical (PEC) biosensor with preponderant sensitivity and anti-interference is a challenge for detecting small molecules in real samples with complex matrices. To this end, the Bi2O3/CuBi2O4 was synthesized in one step to enhance visible light's absorption ability, transferring the interfacial carrier's efficiency, a high-active Z-scheme heterojunction, and a photocathode biosensor was proposed. For the first time, we used the density functional theory to verify a Z-scheme transfer pathway of photogenerated electrons in Bi2O3/CuBi2O4 and the energy band structure of Bi2O3 and CuBi2O4, respectively. Bi2O3/CuBi2O4-based PEC biosensor was developed for competive immunoassay of small molecular, aflatoxin B1 (AFB1) as an example, resulting in a low detection limit of 297.4 fg/mL and a linear range of 1.4 pg/mL-280 ng/mL in urine, water, peanut, and wheat samples. Using spiked experiments, the satisfied repeatability, reproducibility, stability, and specificity of the Bi2O3/CuBi2O4-based PEC biosensor indicated a promise for application in health care, the environment, and food.