[A murine model of local allergic rhinitis].

医学 卵清蛋白 鼻腔给药 免疫学 H&E染色 生理盐水 白细胞介素5 鼻子 免疫球蛋白E 白细胞介素 病理 内科学 细胞因子 抗体 免疫系统 免疫组织化学 外科
作者
Baiwen Chen,Shenhong Qu,Min Li,Linsong Ye,Shaojie Zhang,Taijie Qin,Hua Fan
出处
期刊:PubMed 卷期号:51 (7): 533-7 被引量:5
标识
DOI:10.3760/cma.j.issn.1673-0860.2016.07.010
摘要

To establish the murine models of local allergic rhinitis (LAR) and allergic rhinitis (AR) by using ovalbumin (OVA), and to investigate the relationship between them.Thirty BALB/c mice were divided into 5 groups, (1) the nasally sensitized group (group A1) that was challenged with OVA by a 10 d procedure, (2) the control group of A1 that was challenged with phosphate-buffered saline (PBS), (3) the nasally sensitized group (group A2) that was challenged with OVA by a 25 d procedure, (4)the control group of A2 that was challenged with PBS, (5) the intraperitoneally sensitized group (group B) .The numbers of sneezing after final challenge were counted, and the serum OVA-specific immunoglobulin E (OVA-sIgE), interleukin (IL) -4, IL-13, IL-5 levels in nasal lavage fluid were measured by ELISA. Hematoxylin-eosin staining was performed to evaluate the histological change of nose and lung tissues. Graph Pad Prism 6 software was used to analyze the data.Nasally sensitized group A1 displayed LAR symptoms of sneezing and eosinophilic infiltrating, but without increased OVA-sIgE in serum on day 10 compared with the control group of A1(t=0.697, P>0.05), OVA-sIgE in serum of group A2(2.710±1.406)ng/ml reached to statistical significance and with airway remodeling on day 25 compared with the control group of A2((0.221±0.080)ng/ml, t=4.329, P<0.05). IL-5 and IL-13 in nasal fluid showed a significant increase in the nasally sensitized group A1, compared with the group A2(t values were 2.442, 2.804, P values were less then 0.05).A short time intranasal instillation with OVA could establish LAR murine model, continuing OVA challenge could increase serum sIgE level and with airway remodeling. LAR mice show a unique characteristic by expressing higher IL-5 and IL-13 in nose than AR mice, but sIgE in serum remains at a normal level.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
大地完成签到,获得积分10
刚刚
如意的冰双完成签到 ,获得积分10
1秒前
孤独曲奇完成签到,获得积分10
1秒前
1秒前
乐观的凝梦完成签到,获得积分10
1秒前
丘比特应助菠萝炒蛋加饭采纳,获得10
2秒前
丘比特应助zhenya采纳,获得10
2秒前
2秒前
2秒前
fufu发布了新的文献求助10
2秒前
Ava应助geostar采纳,获得10
2秒前
2秒前
高兴海白发布了新的文献求助10
3秒前
典雅的静发布了新的文献求助10
3秒前
开心浩阑应助Hollow采纳,获得20
3秒前
689完成签到,获得积分20
3秒前
wsy发布了新的文献求助10
4秒前
清爽冬卉完成签到,获得积分10
5秒前
7秒前
summer发布了新的文献求助10
7秒前
ylf发布了新的文献求助20
7秒前
诚心中恶发布了新的文献求助10
7秒前
隐形曼青应助dqq采纳,获得10
8秒前
王鑫发布了新的文献求助10
8秒前
11发布了新的文献求助10
8秒前
YZMING发布了新的文献求助10
8秒前
纯情女大完成签到 ,获得积分10
8秒前
leoleo完成签到,获得积分10
9秒前
云梦泽完成签到,获得积分20
10秒前
冷艳的靖柏完成签到,获得积分10
10秒前
11秒前
丘比特应助现代子默采纳,获得10
11秒前
yanzu发布了新的文献求助20
12秒前
13秒前
顾矜应助XIAOMING采纳,获得10
13秒前
13秒前
13秒前
nissy完成签到,获得积分10
13秒前
ED应助杨立方采纳,获得10
13秒前
13秒前
高分求助中
Ophthalmic Equipment Market by Devices(surgical: vitreorentinal,IOLs,OVDs,contact lens,RGP lens,backflush,diagnostic&monitoring:OCT,actorefractor,keratometer,tonometer,ophthalmoscpe,OVD), End User,Buying Criteria-Global Forecast to2029 2000
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Cognitive Neuroscience: The Biology of the Mind (Sixth Edition) 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 588
Christian Women in Chinese Society: The Anglican Story 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3961465
求助须知:如何正确求助?哪些是违规求助? 3507798
关于积分的说明 11138163
捐赠科研通 3240268
什么是DOI,文献DOI怎么找? 1790870
邀请新用户注册赠送积分活动 872609
科研通“疑难数据库(出版商)”最低求助积分说明 803288