生物
肌发生
RNA剪接
选择性拼接
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起始密码子
核糖核酸
遗传学
环状RNA
细胞生物学
杜氏肌营养不良
肌营养不良蛋白
心肌细胞
计算生物学
信使核糖核酸
基因
肽序列
作者
Ivano Legnini,Gaia Di Timoteo,Francesca Maria Rossi,Mariangela Morlando,Francesca Briganti,Olga Sthandier,Alessandro Fatica,Tiziana Santini,Adrian Andronache,Mark Wade,Pietro Laneve,Nikolaus Rajewsky,Irene Bozzoni
出处
期刊:Molecular Cell
[Elsevier]
日期:2017-04-01
卷期号:66 (1): 22-37.e9
被引量:1632
标识
DOI:10.1016/j.molcel.2017.02.017
摘要
Circular RNAs (circRNAs) constitute a family of transcripts with unique structures and still largely unknown functions. Their biogenesis, which proceeds via a back-splicing reaction, is fairly well characterized, whereas their role in the modulation of physiologically relevant processes is still unclear. Here we performed expression profiling of circRNAs during in vitro differentiation of murine and human myoblasts, and we identified conserved species regulated in myogenesis and altered in Duchenne muscular dystrophy. A high-content functional genomic screen allowed the study of their functional role in muscle differentiation. One of them, circ-ZNF609, resulted in specifically controlling myoblast proliferation. Circ-ZNF609 contains an open reading frame spanning from the start codon, in common with the linear transcript, and terminating at an in-frame STOP codon, created upon circularization. Circ-ZNF609 is associated with heavy polysomes, and it is translated into a protein in a splicing-dependent and cap-independent manner, providing an example of a protein-coding circRNA in eukaryotes.
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