血桂碱
白屈菜红碱
彗星试验
MTT法
遗传毒性
活力测定
细胞周期
细胞毒性
流式细胞术
生物碱
药理学
化学
癌细胞
细胞毒性T细胞
生物
细胞凋亡
传统医学
分子生物学
毒性
医学
癌症
DNA损伤
体外
生物化学
植物
内科学
激酶
蛋白激酶C
DNA
作者
Igor Vivian de Almeida,Liliane Menezes Fernandes,Bruna Isabela Biazi,Verônica Elisa Pimenta Vicentini
出处
期刊:Anti-cancer Agents in Medicinal Chemistry
[Bentham Science]
日期:2017-02-23
卷期号:17 (11)
被引量:16
标识
DOI:10.2174/1871520617666170213115132
摘要
Breast cancer is associated with a high mortality rate around the world due to its aggressiveness and high resistance to conventional therapies. Sanguinarine (SAN) and Chelerythrine (CHE) are plant alkaloids extracted from Sanguinaria canadensis and Macleaya cordata, which have been studied for their bioactivities.To determine the anticancer activities of Sanguinarine (SAN) and Chelerythrine (CHE) plant alkaloids.The MTT assay, the alkaline comet assay and cell cycle analyses by flow cytometry were performed.It was observed that SAN was cytotoxic to human breast adenocarcinoma cells (MCF-7) at concentrations of 7.5 µM (24 and 48 hours), effectively reducing cell viability from the concentration of 10 µM for 24 hours and 7.5 µM for 48 hours, by the MTT test. CHE, in turn, was cytotoxic at concentrations of 10 and 20 µM (48 hours), but did not compromise the cellular viability. The comet assay indicated that SAN was genotoxic to the MCF-7 cells, with a significant increment of damage at 10 µM, while none of the tested concentrations of CHE showed a genotoxic effect. The flow cytometry analysis indicated that no cell cycle arrest was caused by both alkaloids, but SAN 10 µM induced a sub-G1 cell population.The results of cytotoxicity, genotoxicity and cell cycle monitoring that are presented in this paper have suggested that SAN has more of a chemotherapeutic activity, as well as having the potential for the development of new therapies for breast cancer, when compared to CHE.
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