Subchronic nonylphenol exposure induced anxiety-like behavior and decreased expressions of regulators of synaptic plasticity in rats

壬基酚 体内 内科学 内分泌学 化学 神经毒性 海马体 毒性 生物 医学 生物技术 环境化学
作者
Shengnan Li,Weihong Xu,Lei Gong,Jianling Zhang,Yujie Zhang,Jingting Chen,Jie Yu,Jie Xu
出处
期刊:Chemosphere [Elsevier BV]
卷期号:282: 130994-130994 被引量:11
标识
DOI:10.1016/j.chemosphere.2021.130994
摘要

Studies have shown that there were associations between endocrine disrupting chemicals (EDCs) and anxiety. Nonylphenol (NP) is an EDC with weak estrogen activity. This study aimed to clarify whether subchronic exposure of NP at environmental concentrations induces anxiety-like behavior, and effects of NP on the regulators (NMDAR2B, PSD-95, Synapsin1) expressions of synaptic plasticity in vivo and in vitro experiments. In vivo, 40 male SD rats were randomly divided into 4 groups (each with 10 rats): low dose (0.4 mg/kg/day, L-NP), middle-dose (4 mg/kg/day, M − NP), high-dose (40 mg/kg/day, H-NP) and corn oil (Control) groups. In vitro, HT22 cells were divided into a control group (Control), NP group (NP, 20 μM), glutamine acid receptor inhibitor group (MK-801, 10 μM) and MK-801 + NP group. The concentration of NP in the hippocampus rised with the increase of NP exposure concentration in the treatment groups (F = 7.542, P = 0.001). Compared with the control group, the residence time in the dark box after NP exposure had extended (F = 117.927, P < 0.01). The duration (F = 112.054, P < 0.01) and the number of times (F = 13.514, P < 0.01) to enter the closed arm in the NP exposure group significantly increased. There were more neurons degeneration and nuclear shrinkage in the M − and H– NP groups, while the average number of shrinked neurons increased with the increasing dose of NP exposure. The protein expressions of PSD-95 (F = 97.723, P < 0.01), Synapsin1 (F = 41.797, P < 0.01) and NMDAR2B (F = 3.440, P = 0.036) in the NP group were lower than those of the control. Simultaneously, the expressions of PSD-95, Synapsin1 and NMDAR2B in the hippocampus were down-regulated; the mRNA expression of PSD-95 (F = 19.950, P < 0.01), Synapsin1 (F = 3.498, P = 0.035) and NMDAR2B (F = 9.293, P < 0.01) genes in the hippocampus decreased in the M − and H-NP groups. In vitro, the trend of the fluorescence intensity expressed by PSD-95 (F = 2.606, P = 0.124) and Synapsin1 (F = 20.573, P < 0.01) among the groups was: MK-801 + NP group < MK-801 < NP group. The protein expressions of PSD-95 (F = 5.699, P = 0.022), Synapsin1 (F = 10.820, P = 0.003) and NMDAR2B (F = 6.041, P = 0.019) were down-regulated. These results suggested that subchronic exposure to environmental concentrations of NP induced anxiety, and reduced the protein and/or mRNA expressions of regulators of synaptic plasticity (PSD-95, Synapsin1, NMDAR2B).
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
辛未完成签到 ,获得积分10
刚刚
1秒前
牛牛眉目发布了新的文献求助10
3秒前
Dada完成签到,获得积分10
4秒前
RichardBillyham完成签到 ,获得积分10
5秒前
7秒前
kk完成签到 ,获得积分10
7秒前
迷途的羔羊完成签到 ,获得积分10
8秒前
11秒前
阔达芾完成签到,获得积分10
12秒前
12秒前
13秒前
机智的乌完成签到,获得积分10
16秒前
阿里院士完成签到,获得积分10
16秒前
17秒前
牛牛眉目发布了新的文献求助10
19秒前
在写了完成签到,获得积分10
20秒前
爱科研的小多肉完成签到,获得积分10
21秒前
21秒前
cijing发布了新的文献求助10
21秒前
24秒前
李li完成签到,获得积分10
24秒前
holi完成签到 ,获得积分10
25秒前
背后如彤完成签到 ,获得积分10
25秒前
脏zang完成签到,获得积分10
27秒前
1234完成签到,获得积分10
30秒前
Orange应助公孙世往采纳,获得10
30秒前
30秒前
科研通AI2S应助李li采纳,获得10
30秒前
31秒前
煦白发布了新的文献求助30
31秒前
zxy关闭了zxy文献求助
32秒前
32秒前
32秒前
wlgjr发布了新的文献求助10
34秒前
34秒前
35秒前
耍酷的觅荷完成签到 ,获得积分10
37秒前
牛牛眉目发布了新的文献求助10
38秒前
mouxia完成签到,获得积分10
38秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3966366
求助须知:如何正确求助?哪些是违规求助? 3511778
关于积分的说明 11159739
捐赠科研通 3246353
什么是DOI,文献DOI怎么找? 1793415
邀请新用户注册赠送积分活动 874427
科研通“疑难数据库(出版商)”最低求助积分说明 804374