酿酒酵母
生物
乌拉3
RNA剪接
酵母
巢状曲霉
遗传学
抄写(语言学)
发起人
基因
内含子
青霉属
异源表达
乳克鲁维酵母
基因表达
核糖核酸
突变体
哲学
重组DNA
语言学
作者
Zhenquan Lin,Kang Xu,Cai Guang,Yangqingxue Liu,Yi Li,Zhihao Zhang,Jens Nielsen,Shuobo Shi,Zihe Liu
出处
期刊:Journal of Industrial Microbiology & Biotechnology
[Oxford University Press]
日期:2021-08-13
卷期号:48 (9-10)
摘要
Abstract Heterologous expression of eukaryotic gene clusters in yeast has been widely used for producing high-value chemicals and bioactive secondary metabolites. However, eukaryotic transcription cis-elements are still undercharacterized, and the cross-species expression mechanism remains poorly understood. Here we used the whole expression unit (including original promoter, terminator, and open reading frame with introns) of orotidine 5′-monophosphate decarboxylases from 14 Penicillium species as a showcase, and analyzed their cross-species expression in Saccharomyces cerevisiae. We found that pyrG promoters from the Penicillium species could drive URA3 expression in yeast, and that inefficient cross-species splicing of Penicillium introns might result in weak cross-species expression. Thus, this study demonstrates cross-species expression from Penicillium to yeast, and sheds light on the opportunities and challenges of cross-species expression of fungi expression units and gene clusters in yeast without refactoring for novel natural product discovery.
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