细胞
核糖核酸
单细胞分析
生物
细胞生物学
化学
计算生物学
基因
生物化学
作者
Francis Shue,Francis Shue,Yu Yamazaki,Yuka A. Martens,Guojun Bu,Chia‐Chen Liu
出处
期刊:STAR protocols
[Elsevier]
日期:2021-09-01
卷期号:2 (3): 100715-100715
被引量:3
标识
DOI:10.1016/j.xpro.2021.100715
摘要
Cerebral blood vessels supply oxygen and nutrients, remove metabolic waste, and play a critical role in maintaining brain homeostasis. Cerebrovasculature is composed of heterogeneous populations of brain vascular cells (BVCs). A major challenge in effective cerebrovascular transcriptional profiling is high-quality BVC procurement, permitting high sequencing depth. Here, we establish cell isolation procedures for glio-vascular cell-enriched single-cell RNA sequencing enabling unbiased characterization of BVC transcriptional heterogeneity. Our approach can be used to address vascular-specific contribution to brain diseases. For complete details on the use and execution of this protocol, please refer to Yamazaki et al. (2021).
科研通智能强力驱动
Strongly Powered by AbleSci AI