Preparation of dioscin from Trigonella foenum-graecum by foam separation–preparative high-performance liquid chromatography

Dioscin in Trigonella foenum-graecum was purified by foam separation combined with preparative high-performance liquid chromatography (Prep-HPLC) and the preparation method of dioscin monomer was established. Then, the structure was confirmed by FI-IR, HRMS, 13C-NMR and 1H-NMR.The results showed that the method had the advantages of simple operation, short separation period and high efficiency and was suitable for the separation and preparation of dioscin from Trigonella foenum-graecum.