•Biomechanics of orthodontic aligners are studied in vitro. •Maxillary central incisor, canine, and second premolar movements were considered. •Forces and moments were measured for displaced and adjacent teeth. •The position of the displaced tooth around the arch was found to be of significance. •Forces and moments acting on adjacent teeth were found to be clinically significant. Introduction The objective of this study was to evaluate the forces and moments exerted by orthodontic aligners on 3 different displaced maxillary teeth and their adjacent supporting teeth. Methods An in vitro orthodontic simulator was used to measure the forces and moments of a 0.75-mm thick glycol-modified polyethylene terephthalate material for 3 maxillary teeth: central incisor, canine, and second premolar. Forces and moments were recorded for tested teeth displaced lingually one by one for 0.20 mm. Repeated measures of multivariate analysis of variance was used to assess the outcome. Results The mean buccolingual force applied on a displaced canine (2.25 ± 0.38 N) was significantly (P <0.001) more than the central incisor (1.49 ± 0.18 N) and second premolar (1.50 ± 0.16 N). The mean moment (that tends to tip the teeth buccally) exerted on a canine (−20.11 ± 5.27 Nmm) was significantly more (P <0.001) than the central incisor (−8.42 ± 1.67 Nmm) and second premolar (−11.45 ± 1.29 Nmm). The forces and moments acting on teeth adjacent to the displaced tooth were clinically significant and acted in opposing directions to those on the displaced tooth. Conclusions The results of this study highlighted that for the same amount of displacement on a given tooth, the forces and moments imposed by the orthodontic aligner depend on location around the arch. These findings highlight the need to further study aligner mechanics around the dental arch and optimize aligner design to impose desired mechanical loads to avoid detrimental effects during orthodontic tooth movement. The objective of this study was to evaluate the forces and moments exerted by orthodontic aligners on 3 different displaced maxillary teeth and their adjacent supporting teeth. An in vitro orthodontic simulator was used to measure the forces and moments of a 0.75-mm thick glycol-modified polyethylene terephthalate material for 3 maxillary teeth: central incisor, canine, and second premolar. Forces and moments were recorded for tested teeth displaced lingually one by one for 0.20 mm. Repeated measures of multivariate analysis of variance was used to assess the outcome. The mean buccolingual force applied on a displaced canine (2.25 ± 0.38 N) was significantly (P <0.001) more than the central incisor (1.49 ± 0.18 N) and second premolar (1.50 ± 0.16 N). The mean moment (that tends to tip the teeth buccally) exerted on a canine (−20.11 ± 5.27 Nmm) was significantly more (P <0.001) than the central incisor (−8.42 ± 1.67 Nmm) and second premolar (−11.45 ± 1.29 Nmm). The forces and moments acting on teeth adjacent to the displaced tooth were clinically significant and acted in opposing directions to those on the displaced tooth. The results of this study highlighted that for the same amount of displacement on a given tooth, the forces and moments imposed by the orthodontic aligner depend on location around the arch. These findings highlight the need to further study aligner mechanics around the dental arch and optimize aligner design to impose desired mechanical loads to avoid detrimental effects during orthodontic tooth movement.