Velvet antler polypeptide-loaded polyvinyl alcohol-sodium alginate hydrogels promote the differentiation of neural progenitor cells in 3D towards oligodendrocytes in vitro

Three-dimensional (3D) culture of neural progenitor cells (NPCs) in hydrogels represents a powerful means for recapitulating neurodevelopment, disease modelling and drug discovery. However, the differentiation of NPCs to oligodendrocytes in 3D scaffolds remains a great challenge. In this study, polyvinyl alcohol (PVA) - sodium alginate (SA) composite hydrogels intended for NPC culture in 3D were fabricated by ionic crosslinking between SA and calcium ions. It was demonstrated that adding PVA to the composite hydrogels resulted in increases in pore size and swelling rate and decreases in elastic moduli as the PVA proportion was enhanced. In addition, the composite hydrogels were biocompatible with mouse NPCs and improved the proliferation of the encapsulated NPCs compared with SA hydrogels. Moreover, when velvet antler polypeptides (VAPs), which were capable of facilitating the differentiation of NPCs to oligodendrocyte fate in 2D, were loaded into PVA-SA hydrogels, NPCs differentiated into neurons, astrocytes and oligodendrocytes, with the presence of VAPs promoting oligodendrogenesis in a dose-dependant manner. The present composite hydrogels provide a suitable scaffold for the construction of neural tissue engineering and neurological disease modelling.