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Down-regulation of aflatoxin biosynthetic genes in Aspergillus parasiticus by Heliopsis longipes roots and affinin for reduction of aflatoxin production

黄曲霉毒素 寄生曲霉 生物合成 真菌毒素 基因 曲霉 生物 调节基因 生物化学 黄曲霉 基因簇 基因表达 微生物学 食品科学
作者
Génesis V. Buitimea-Cantúa,Elisa Magaña-Barajas,Nydia E. Buitimea‐Cantúa,Héctor Manuel Leija Gutiérrez,María del Refugio Rocha-Pizaña,Ema Carina Rosas‐Burgos,Alejandro Hernández‐Morales,Jorge Molina‐Torres
出处
期刊:Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes [Taylor & Francis]
卷期号:56 (10): 899-908 被引量:1
标识
DOI:10.1080/03601234.2021.1974273
摘要

Affinin present in Heliopsis longipes roots has been identified as an anti-aflatoxin molecule. However, its mechanism of action has yet to be clarified. Aflatoxins biosynthesis involves not less than 27 enzymatic reactions. In this work, the genes aflG, aflH, aflI, aflK, aflL, aflM, aflO, aflP, and aflQ of the aflatoxins cluster and the aflS gene encoding an internal regulatory factor involved in aflatoxins biosynthesis in Aspergillus parasiticus, were studied by qRT-PCR. Results demonstrated that ethanolic extract of H. longipes roots and affinin inhibit aflatoxin biosynthesis and fungal growth in a dose-dependent manner. At 300 µg/mL, ethanolic extract and affinin presented the highest inhibition of radial growth (86% and 94%) and aflatoxin production (68% and 80%). The qRT-PCR analysis demonstrated that nine tested genes were down-regulated by affinin and ethanolic extract. The most down-regulated was the aflK, a gene that encodes an enzyme cyclase with double function during the aflatoxin biosynthesis. While no significant down-regulation was obtaining for aflH gene. Exposure to affinin also resulted in decreased transcript levels of the internal regulator factor aflS. Based on our results, a model showing the regulatory mechanism in aflatoxin biosynthesis and its role in gene expression was proposed. In conclusion, affinin modulates the expression of several aflatoxin biosynthetic genes, leading to mycotoxin biosynthesis inhibition. Therefore, H. longipes roots is a suitable candidate to developed control strategies via lowering gene expressions as a future perspective in reducing aflatoxin contamination.
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