Down-regulation of aflatoxin biosynthetic genes in Aspergillus parasiticus by Heliopsis longipes roots and affinin for reduction of aflatoxin production

黄曲霉毒素 寄生曲霉 生物合成 真菌毒素 基因 曲霉 生物 调节基因 生物化学 黄曲霉 基因簇 基因表达 微生物学 食品科学
作者
Génesis V. Buitimea-Cantúa,Elisa Magaña-Barajas,Nydia E. Buitimea‐Cantúa,Héctor Manuel Leija Gutiérrez,María del Refugio Rocha-Pizaña,Ema Carina Rosas‐Burgos,Alejandro Hernández‐Morales,Jorge Molina‐Torres
出处
期刊:Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes [Taylor & Francis]
卷期号:56 (10): 899-908 被引量:1
标识
DOI:10.1080/03601234.2021.1974273
摘要

Affinin present in Heliopsis longipes roots has been identified as an anti-aflatoxin molecule. However, its mechanism of action has yet to be clarified. Aflatoxins biosynthesis involves not less than 27 enzymatic reactions. In this work, the genes aflG, aflH, aflI, aflK, aflL, aflM, aflO, aflP, and aflQ of the aflatoxins cluster and the aflS gene encoding an internal regulatory factor involved in aflatoxins biosynthesis in Aspergillus parasiticus, were studied by qRT-PCR. Results demonstrated that ethanolic extract of H. longipes roots and affinin inhibit aflatoxin biosynthesis and fungal growth in a dose-dependent manner. At 300 µg/mL, ethanolic extract and affinin presented the highest inhibition of radial growth (86% and 94%) and aflatoxin production (68% and 80%). The qRT-PCR analysis demonstrated that nine tested genes were down-regulated by affinin and ethanolic extract. The most down-regulated was the aflK, a gene that encodes an enzyme cyclase with double function during the aflatoxin biosynthesis. While no significant down-regulation was obtaining for aflH gene. Exposure to affinin also resulted in decreased transcript levels of the internal regulator factor aflS. Based on our results, a model showing the regulatory mechanism in aflatoxin biosynthesis and its role in gene expression was proposed. In conclusion, affinin modulates the expression of several aflatoxin biosynthetic genes, leading to mycotoxin biosynthesis inhibition. Therefore, H. longipes roots is a suitable candidate to developed control strategies via lowering gene expressions as a future perspective in reducing aflatoxin contamination.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
程院发布了新的文献求助10
2秒前
3秒前
无私代芹发布了新的文献求助10
5秒前
江梦曼完成签到,获得积分10
6秒前
量子星尘发布了新的文献求助10
8秒前
吕老黄完成签到,获得积分10
8秒前
9秒前
Fushuai完成签到,获得积分10
9秒前
田様应助jbhb采纳,获得10
9秒前
轩辕寄风发布了新的文献求助10
9秒前
南风完成签到,获得积分10
11秒前
大模型应助老迟到的惋清采纳,获得10
12秒前
小崔加油发布了新的文献求助10
12秒前
zhongjiaweiv完成签到,获得积分20
13秒前
顾矜应助Mars采纳,获得10
15秒前
香蕉觅云应助无限的可乐采纳,获得20
15秒前
15秒前
16秒前
16秒前
李英豪应助香菜采纳,获得10
17秒前
虚拟的傲玉完成签到,获得积分10
18秒前
在水一方应助zhongjiaweiv采纳,获得10
18秒前
20秒前
如初发布了新的文献求助10
20秒前
轩辕寄风发布了新的文献求助10
20秒前
23秒前
23秒前
斯文败类应助huayi采纳,获得10
24秒前
dcc发布了新的文献求助10
24秒前
25秒前
jbhb发布了新的文献求助10
25秒前
26秒前
高源伯完成签到 ,获得积分10
28秒前
alala发布了新的文献求助10
28秒前
29秒前
30秒前
没有发布了新的文献求助10
31秒前
31秒前
31秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3971670
求助须知:如何正确求助?哪些是违规求助? 3516348
关于积分的说明 11182142
捐赠科研通 3251567
什么是DOI,文献DOI怎么找? 1795907
邀请新用户注册赠送积分活动 876155
科研通“疑难数据库(出版商)”最低求助积分说明 805318