表位
生物
人类白细胞抗原
病毒学
免疫
细胞免疫
免疫系统
CD8型
抗原
免疫学
冠状病毒
疾病
T细胞
2019年冠状病毒病(COVID-19)
传染病(医学专业)
医学
病理
作者
Hang Zhang,Shasha Deng,Liting Ren,Peiyi Zheng,Xiaowen Hu,Tengchuan Jin,Xu Tan
出处
期刊:Cell Reports
[Elsevier]
日期:2021-08-27
卷期号:36 (11): 109708-109708
被引量:50
标识
DOI:10.1016/j.celrep.2021.109708
摘要
Cellular immunity is important in determining the disease severity of COVID-19 patients. However, current understanding of SARS-CoV-2 epitopes mediating cellular immunity is limited. Here we apply T-Scan, a recently developed method, to identify CD8+ T cell epitopes from COVID-19 patients of four major HLA-A alleles. Several identified epitopes are conserved across human coronaviruses, which might mediate pre-existing cellular immunity to SARS-CoV-2. In addition, we identify and validate four epitopes that were mutated in the newly circulating variants, including the Delta variant. The mutations significantly reduce T cell responses to the epitope peptides in convalescent and vaccinated samples. We further determine the crystal structure of HLA-A∗02:01/HLA-A∗24:02 in complex with the epitope KIA_S/NYN_S, respectively, which reveals the importance of K417 and L452 of the spike protein for binding to HLA. Our data suggest that evading cellular immunity might contribute to the increased transmissibility and disease severity associated with the new SARS-CoV-2 variants.
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