Procedures for Fecal Microbiota Transplantation in Murine Microbiome Studies

粪便细菌疗法 微生物群 失调 移植 肠道菌群 疾病 粪便 生物 免疫学 医学 抗生素 生物信息学 艰难梭菌 微生物学 内科学
作者
Suresh Bokoliya,Yair Dorsett,Hunter Panier,Yanjiao Zhou
出处
期刊:Frontiers in Cellular and Infection Microbiology [Frontiers Media SA]
卷期号:11 被引量:97
标识
DOI:10.3389/fcimb.2021.711055
摘要

Fecal microbiota transplantation (FMT) has been widely recognized as an approach to determine the microbiome’s causal role in gut dysbiosis-related disease models and as a novel disease-modifying therapy. Despite potential beneficial FMT results in various disease models, there is a variation and complexity in procedural agreement among research groups for performing FMT. The viability of the microbiome in feces and its successful transfer depends on various aspects of donors, recipients, and lab settings. This review focuses on the technical practices of FMT in animal studies. We first document crucial factors required for collecting, handling, and processing donor fecal microbiota for FMT. Then, we detail the description of gut microbiota depletion methods, FMT dosages, and routes of FMT administrations in recipients. In the end, we describe assessments of success rates of FMT with sustainability. It is critical to work under the anaerobic condition to preserve as much of the viability of bacteria. Utilization of germ- free mice or depletion of recipient gut microbiota by antibiotics or polyethylene glycol are two common recipient preparation approaches to achieve better engraftment. Oral-gastric gavage preferred by most researchers for fast and effective administration of FMT in mice. Overall, this review highlights various methods that may lead to developing the standard and reproducible protocol for FMT.
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